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Syntaxin 1a antibody

Anti-Syntaxin 1a antibody used in Western Blot (WB). GTX113559
Anti-Syntaxin 1a antibody used in IHC (Paraffin sections) (IHC-P). GTX113559
Anti-Syntaxin 1a antibody used in Western Blot (WB). GTX113559
Anti-Syntaxin 1a antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113559

Cat. No. GTX113559

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Syntaxin 1a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain

Predict Reactivity

Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human Syntaxin 1a. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620122

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

syntaxin 1A , HPC-1 , P35-1 , STX1 , SYN1A

Cellular Localization

Cell membrane; Single-pass type IV membrane protein , Isoform 2: Secreted

Background

Synaptic vesicles store neurotransmitters that are released during calcium-regulated exocytosis. The specificity of neurotransmitter release requires the localization of both synaptic vesicles and calcium channels to the presynaptic active zone. Syntaxins function in this vesicle fusion process. Syntaxins also serve as a substrate for botulinum neurotoxin type C, a metalloprotease that blocks exocytosis and has high affinity for a molecular complex that includes the alpha-latrotoxin receptor (MIM 600565) which produces explosive exocytosis (Zhang et al., 1995 [PubMed 7622072]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Syntaxin 1a antibody used in Western Blot (WB). GTX113559

GTX113559 WB Image

Various whole cell extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntaxin 1a antibody (GTX113559) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Syntaxin 1a antibody used in IHC (Paraffin sections) (IHC-P). GTX113559

GTX113559 IHC-P Image

Syntaxin 1a antibody detects Syntaxin 1a protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Syntaxin 1a antibody (GTX113559) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Syntaxin 1a antibody used in Western Blot (WB). GTX113559

GTX113559 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntaxin 1a antibody (GTX113559) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Syntaxin 1a antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113559

GTX113559 ICC/IF Image

Syntaxin 1a antibody detects Syntaxin 1a protein at cell body and synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Syntaxin 1a protein stained by Syntaxin 1a antibody (GTX113559) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Lu Z et al. Mol Ther 2022; 30 (6) : 2163-2175 Base-edited cynomolgus monkeys mimic core symptoms of STXBP1 encephalopathy.
Perluigi M et al. Alzheimers Dement 2021; Aberrant crosstalk between insulin signaling and mTOR in young Down syndrome individuals revealed by neuronal-derived extracellular vesicles.
Application : WB
Reactivity : Human
Kuge H et al. J Lipid Res 2020; 61 (12) : 1747-1763 PLRP2 selectively localizes synaptic membrane proteins via acyl-chain remodeling of phospholipids.
Application : ICC/IF
Reactivity : Rat
Tramutola A et al. Neurobiol Dis 2020; 137 : 104772 Brain insulin resistance triggers early onset Alzheimer disease in Down syndrome.
Application : WB
Reactivity : Human
Hausrat TJ et al. Nat Commun 2015; 6 : 6872 Radixin regulates synaptic GABAA receptor density and is essential for reversal learning and short-term memory.
Application : WB
Reactivity : Mouse
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169