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PSMA6 antibody

Anti-PSMA6 antibody used in IHC (Paraffin sections) (IHC-P). GTX113530
Anti-PSMA6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113530
Anti-PSMA6 antibody used in IHC (Paraffin sections) (IHC-P). GTX113530
Anti-PSMA6 antibody used in Western Blot (WB). GTX113530
Anti-PSMA6 antibody used in IHC (Paraffin sections) (IHC-P). GTX113530
Anti-PSMA6 antibody used in Western Blot (WB). GTX113530
Anti-PSMA6 antibody used in Western Blot (WB). GTX113530
Anti-PSMA6 antibody used in IHC (Paraffin sections) (IHC-P). GTX113530

Cat. No. GTX113530

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PSMA6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , mouse liver , rat liver

Predict Reactivity

Sheep, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PSMA6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037764

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proteasome subunit alpha 6 , IOTA , PROS27 , p27K

Cellular Localization

Cytoplasm , Nucleus

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. A pseudogene has been identified on the Y chromosome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PSMA6 antibody used in IHC (Paraffin sections) (IHC-P). GTX113530

GTX113530 IHC-P Image

Proteasome 20S alpha 6 antibody detects Proteasome 20S alpha 6 protein at cytosol and nucleus on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Proteasome 20S alpha 6 antibody (GTX113530) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PSMA6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113530

GTX113530 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using 20S Proteasome alpha6 (GTX113530) antibody at 1:200 dilution.

Anti-PSMA6 antibody used in IHC (Paraffin sections) (IHC-P). GTX113530

GTX113530 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft , using 20S Proteasome alpha6 (GTX113530) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PSMA6 antibody used in Western Blot (WB). GTX113530

GTX113530 WB Image

Proteasome 20S alpha 6 antibody detects PSMA6 protein by Western blot analysis.
A. 50 μg rat liver lysate/extract
12 % SDS-PAGE
Proteasome 20S alpha 6 antibody (GTX113530) dilution: 1:1000

Anti-PSMA6 antibody used in IHC (Paraffin sections) (IHC-P). GTX113530

GTX113530 IHC-P Image

Proteasome 20S alpha 6 antibody detects Proteasome 20S alpha 6 protein at cytosol and nucleus on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
Proteasome 20S alpha 6 antibody (GTX113530) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PSMA6 antibody used in Western Blot (WB). GTX113530

GTX113530 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: Hela
12% SDS PAGE
GTX113530 diluted at 1:1000

Anti-PSMA6 antibody used in Western Blot (WB). GTX113530

GTX113530 WB Image

Proteasome 20S alpha 6 antibody detects PSMA6 protein by Western blot analysis.
A. 50 μg mouse liver lysate/extract
12 % SDS-PAGE
Proteasome 20S alpha 6 antibody (GTX113530) dilution: 1:1000

Anti-PSMA6 antibody used in IHC (Paraffin sections) (IHC-P). GTX113530

GTX113530 IHC-P Image

Proteasome 20S alpha 6 antibody detects Proteasome 20S alpha 6 protein at cytosol and nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Proteasome 20S alpha 6 antibody (GTX113530) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Deglincerti A et al. Nat Commun 2015; 6 : 6888 Coupled local translation and degradation regulate growth cone collapse.
Application : ICC/IF
Reactivity : Rat
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169