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VCP antibody [N1N2], N-term

Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in IHC (Whole mount) (IHC-Wm). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030
Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

Cat. No. GTX113030

Reference 27
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Rat, Zebrafish, Dog
Package
100 μl ($399),
25 μl ($169)
See all VCP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

Mouse brain , rat brain , A549 , H1299 , HCT116

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human VCP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952542

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

valosin containing protein , CDC48 , TERA , p97

Cellular Localization

Cytoplasm , cytosol , Nucleus

Background

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030

GTX113030 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using VCP antibody [N1N2], N-term (GTX113030) at 1:300 dilution.

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody [N1N2], N-term (GTX113030) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VCP antibody [N1N2], N-term used in IHC (Whole mount) (IHC-Wm). GTX113030

GTX113030 IHC-Wm Image

VCP antibody [N1N2], N-term detects Vcp protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
VCP antibody [N1N2], N-term (GTX113030) dilution: 1:100.
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

VCP antibody detects VCP protein by Western blot analysis.
A. 50 μg rat brain lysate/extract
7.5 % SDS-PAGE
VCP antibody (GTX113030) dilution: 1:1000

Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030

GTX113030 IHC-P Image

VCP antibody [N1N2], N-term detects VCP protein at cytoplasm and nucleus in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
VCP antibody [N1N2], N-term (GTX113030) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody [N1N2], N-term (GTX113030) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030

GTX113030 IHC-P Image

VCP antibody [N1N2], N-term detects VCP protein at nucleus and cytosol on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
VCP antibody [N1N2], N-term (GTX113030) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030

GTX113030 IHC-P Image

VCP antibody [N1N2], N-term detects VCP protein at cytoplasm and nucleus in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
VCP antibody [N1N2], N-term (GTX113030) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX113030 diluted at 1:5000

Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030

GTX113030 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using VCP(p97)(GTX113030) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

The data was published in the journal FEBS Open Bio in 2018.PMID: 30186749

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

The data was published in the journal FEBS Open Bio in 2018.PMID: 30186749

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

The data was published in the journal FEBS Open Bio in 2018.PMID: 30186749

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

The data was published in the journal PLoS One in 2015. PMID: 26678046

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

The data was published in the journal FEBS Open Bio in 2018.PMID: 30186749

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

The data was published in the 2022 in BMC Cancer. PMID: 35277124

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

GTX113030 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28655918

REFERENCE

Application Reference

Kitamura N et al. J Vet Med Sci 2023; 85 (12) : 1319-1323 Protein phosphatase 6 promotes transforming growth factor-β signaling in mouse embryonic fibroblasts.
Kohyanagi N et al. J Biol Chem 2023; 300 (1) : 105584 PP2A inhibitor SET promotes mTORC1 and Bmi1 signaling through Akt activation and maintains the colony-formation ability of cancer cells.
Yamamoto M et al. J Vet Med Sci 2023; 85 (9) : 977-984 Protein phosphatase 6 regulates trametinib sensitivity, a mitogen-activated protein kinase kinase (MEK) inhibitor, by regulating MEK1/2-ERK1/2 signaling in canine melanoma cells.
Yomogi Shiota et al. Biomed Pharmacother 2023; 165 : 115079 Derivation of a new model of lung adenocarcinoma using canine lung cancer organoids for translational research in pulmonary medicine
Application : WB
Hancock JL et al. J Exp Clin Cancer Res 2023; 42 (1) : 90 COMMD3 loss drives invasive breast cancer growth by modulating copper homeostasis.
Application : WB
Reactivity : Human
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Submit a Reference

REVIEW

VCP antibody [N1N2], N-term Cat. No. GTX113030

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030
Date : Anonymous submitted on 21-Jan-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Primary Antibody : 1:20000, 4°C, 16Hr
Submit a Review
Application Reference
Kitamura N et al. J Vet Med Sci 2023; 85 (12):1319-1323 Protein phosphatase 6 promotes transforming growth factor-β signaling in mouse embryonic fibroblasts.
Kohyanagi N et al. J Biol Chem 2023; 300 (1):105584 PP2A inhibitor SET promotes mTORC1 and Bmi1 signaling through Akt activation and maintains the colony-formation ability of cancer cells.
Yamamoto M et al. J Vet Med Sci 2023; 85 (9):977-984 Protein phosphatase 6 regulates trametinib sensitivity, a mitogen-activated protein kinase kinase (MEK) inhibitor, by regulating MEK1/2-ERK1/2 signaling in canine melanoma cells.
Yomogi Shiota et al. Biomed Pharmacother 2023; 165:115079 Derivation of a new model of lung adenocarcinoma using canine lung cancer organoids for translational research in pulmonary medicine
Application : WB
Hancock JL et al. J Exp Clin Cancer Res 2023; 42 (1):90 COMMD3 loss drives invasive breast cancer growth by modulating copper homeostasis.
Application : WB
Reactivity : Human
Guettsches AK et al. Biomedicines 2022; 10 (10) FYCO1 Increase and Effect of Arimoclomol-Treatment in Human VCP-Pathology.
Reactivity : Human
Ryouichi Tsunedomi et al. BMC Cancer 2022; 22 (1):260 Elevated expression of RAB3B plays important roles in chemoresistance and metastatic potential of hepatoma cells.
Application : WB
Reactivity : Human
Kitamura N et al. J Biochem 2021; 170 (1):131-138 Protein phosphatase 6 promotes neurite outgrowth by promoting mTORC2 activity in N2a cells.
Fujiwara N et al. Biochem Biophys Res Commun 2021; 552:191-195 Protein phosphatase 6 dissociates the Beclin 1/Vps34 complex and inhibits autophagy.
Fujiwara Y et al. Pancreas 2021; 50 (3):405-413 Pancreatic Cancer Stem-Like Cells With High Calreticulin Expression Associated With Immune Surveillance.
Application : WB
Reactivity : Human
Fujimoto T et al. Oncol Lett 2021; 21 (1):30 Cathepsin B is highly expressed in pancreatic cancer stem-like cells and is associated with patients' surgical outcomes.
Application : WB
Reactivity : Human
Fujiwara N et al. Cancer Sci 2020; 111 (12):4371-4380 Autophagy regulates levels of tumor suppressor enzyme protein phosphatase 6.
Application : WB
Reactivity : Human
Ikeda S et al. J Biochem 2020; Involvement of PP2A Methylation in the Adipogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cell.
Application : WB
Reactivity : Mouse
Tsuji S et al. J Vet Med Sci 2019; Efficacy of an anti-cancer strategy targeting SET in canine osteosarcoma.
Application : WB
Reactivity : Dog
Chernis N et al. Am J Physiol Cell Physiol 2019; Bisphenol AF promotes inflammation in human white adipocytes.
Application : WB
Reactivity : Human
Yabe R et al. FEBS Open Bio 2018; 8 (9):1486-1496 A stable association with PME-1 may be dispensable for PP2A demethylation - implications for the detection of PP2A methylation and immunoprecipitation.
Ryotaro Yabe et al. FEBS openbio 2018; (Epub) A stable association with PME-1 may be dispensable for PP2A demethylation: Implications for the detection of PP2A methylation and immunoprecipitation.
Application : WB
Reactivity : Mouse
Koh EH et al. Diabetes 2018; (Epub) mir-30a remodels subcutaneous adipose tissue inflammation to improve * sensitivity in obesity.
Application : WB
Reactivity : Mouse
Kake S et al. Sci Rep 2017; 7 (1):4279 The role of SET/I2PP2A in canine mammary tumors.
Application : WB
Reactivity : Dog
Kake S et al. Oncol Rep 2017; 37 (2):1100-1106 Death-associated protein kinase 3 controls the tumor progression of A549 cells through ERK MAPK/c-Myc signaling.
Reactivity : Human
Fujiwara N et al. J Biol Chem 2016; 291 (20):10858-66 Regulation of Beclin 1 Protein Phosphorylation and Autophagy by Protein Phosphatase 2A (PP2A) and Death-associated Protein Kinase 3 (DAPK3).
Application : WB
Reactivity : Mouse
Tsuji S et al. J Vet Med Sci 2016; (Epub) Anti-tumor effects of * on canine lymphoma.
Application : WB
Reactivity : Dog
Enjoji S et al. J Vet Med Sci 2015; 77 (11):1451-6 The therapeutic effects of SET/I2PP2A inhibitors on canine melanoma.
Application : WB
Reactivity : Dog
Yabe R et al. PLoS One 2015; 10 (12):e0145226 Protein Phosphatase Methyl-Esterase PME-1 Protects Protein Phosphatase 2A from Ubiquitin/Proteasome Degradation.
Application : WB
Reactivity : Mouse
May EW et al. Pharmacol Res 2014; 90C:43117 identification of up- and down-regulated proteins in *-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of *-associated resistance.
Application : WB
Reactivity : Human
Huang CH et al. J Biol Chem 2014; 289 (7):4444-54 Role of HERP and a HERP-related protein in HRD1-dependent protein degradation at the endoplasmic reticulum.
Application : WB
Reactivity : Human
Huang CH et al. J Biol Chem 2013; 288 (35):25330-9 Derlin2 protein facilitates HRD1-mediated retro-translocation of sonic hedgehog at the endoplasmic reticulum.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169