PRODUCT
Summary
beta-Actin antibody detects beta-actin, a cytoskeletal protein with a predicted molecular weight of 42 kDa. Because of its widespread expression, beta-actin is commonly used as a loading control for various protein assays, particularly western blots, to estimate sample protein amount and quality.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:20000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , Jurkat , Raji , mouse kidney , mouse lung , mouse testis , Candida albicans (sc5314) , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , Rat-2 , NTH-3T3 , mouse brain , PC-12 , Rat brain
Product Note
IP/MS validation was supported by references (PMID:30377395)
Predict Reactivity
Rabbit, Bovine, Guinea pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.4 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949572
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
actin beta , BRWS1 , PS1TP5BP1
Cellular Localization
Cytoplasm , cytoskeleton
Background
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX109639 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX109639) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX109639 WB Image
Sample (30 μg of whole cell lysate) A: 293T B: NTH-3T3 C: mouse brain D: PC-12 E: Rat brain F: drosophila 10% SDS PAGE GTX109639 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX109639 IHC-P Image
beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.beta Actin stained by beta Actin antibody (GTX109639) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX109639 WB Image
Sample (whole cell lysate) A: Candida albicans (sc5314) 20ug B: Candida albicans (sc5314) 40ug 10% SDS PAGE GTX109639 diluted at 1:4000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX109639 WB Image
beta Actin antibody detects ACTB protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat-2 whole cell lysate/extract 10% SDS-PAGE beta Actin antibody (GTX109639) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX109639 ICC/IF Image
beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: beta Actin stained by beta Actin antibody (GTX109639) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX109639 WB Image
beta Actin antibody detects ACTB protein by western blot analysis. A. 50 μg mouse brain lysate/extract B. 50 μg mouse kidney lysate/extract C. 50 μg mouse lung lysate/extract D. 50 μg mouse testis lysate/extract 10% SDS-PAGE beta Actin antibody (GTX109639) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX109639 WB Image
Sample (10 μg of whole cell lysate) A: Yeast lysate 7.5% SDS PAGE GTX109639 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
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GTX109639 IHC-P Image
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Beta actin(GTX109639) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
GTX109639 WB Image
Various whole cell extracts (30 μg) were separated by -% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX109639) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX109639 WB Image
Sample (30 μg of whole cell lysate) A: 293T B: A431 C: Jurkat D: Raji 10% SDS PAGE GTX109639 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX109639 IHC-P Image
beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat liver.beta Actin stained by beta Actin antibody (GTX109639) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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|
GTX109639 WB Image
Sample (30 μg of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG 10% SDS PAGE GTX109639 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX109639 IHC-P Image
beta Actin antibody detects beta Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.beta Actin stained by beta Actin antibody (GTX109639) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX109639 WB Image
Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX109639) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
REVIEW
beta Actin antibody
Cat. No. GTX109639
Rating
|
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( Average 5 based on 95 users reviews)
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Application
Western Blot(WB)
|
|
( Average 5 based on 93 users reviews)
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IHC (Paraffin sections)(IHC-P)
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( Average 5 based on 1 users reviews)
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IHC (Frozen sections)(IHC-Fr)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 24-Jul-2023
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the 2022 in Nutrients.
PMID: 35684095
Date :
Anonymous submitted on 24-Jul-2023
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the 2022 in Elife.
PMID: 35416774
Date :
Anonymous submitted on 24-Jul-2023
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the 2020 in PLoS One.
PMID: 33382817
Date :
Anonymous submitted on 24-Jul-2023
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the 2020 in Antioxidants (Basel).
PMID: 33203103
Date :
Anonymous submitted on 24-Jul-2023
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the 2020 in Antioxidants (Basel).
PMID: 33203103
Date :
Anonymous submitted on 24-Jul-2023
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the 2020 in Neural Plast.
PMID: 33082778
Date :
Anonymous submitted on 24-Jul-2023
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the 2020 in Neural Plast.
PMID: 33082778
Date :
Anonymous submitted on 24-Jul-2023
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the 2020 in Neural Plast.
PMID: 33082778
Date :
Anonymous submitted on 24-Jul-2023
Application Tested :
WB
Sample Species :
Mk, Mosq
Figure Note :
The data was peer reviewed and published in the 2020 in Viruses.
PMID: 33003584
Date :
Anonymous submitted on 24-Jul-2023
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the 2019 in Cancers (Basel).
PMID: 31861872
Date :
Anonymous submitted on 24-Jul-2023
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the 2019 in Cancers (Basel).
PMID: 31861872
Date :
Anonymous submitted on 06-Feb-2022
Application Tested :
IHC-Fr
Sample Species :
Ms
Sample :
Mouse small intestine tissue
Blocking :
1% FBS and 0.4% TritonX-100 in PBS, 25°C, 1Hr
Fixation :
4% PFA in PBS
Primary Antibody :
1:200, 4°C, 16Hr
Date :
Anonymous submitted on 22-Sep-2021
Application Tested :
IHC-P
Sample Species :
Chk
Sample :
Chicken Ileum
Blocking :
10% normal goat serum, 20min, 25°C
Fixation :
Bouin’s fixative
Primary Antibody :
1:5000, 25°C, 24Hr
Figure Note :
Microvilli showing beta-actin immunoreactivity (arrows) are found in the apical surface of enterocytes in the chicken ileum.
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Nat Commun in 2019.
PMID: 31409793
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Cell Death Dis in 2016.
PMID: 27831565
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal Int J Mol Sci in 2019.
PMID: 31744099
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Oncogenesis in 2016.
PMID: 27918551
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal BMC Nephrol in 2018.
PMID: 30005632
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Sci Rep in 2017.
PMID: 28169314
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal J Clin Med in 2019.
PMID: 31167519
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Sci Rep in 2019.
PMID: 31501500
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Exp Ther Med in 2014.
PMID: 24396418
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Sci Rep in 2020.
PMID: 32286426
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal Int J Mol Sci in 2019.
PMID: 31744099
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal J Int Soc Sports Nutr in 2015.
PMID: 25792976
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal J Biomed Sci in 2018.
PMID: 29361943
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Aging Dis in 2019.
PMID: 31164994
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Oncogenesis in 2016.
PMID: 27918551
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Aging Dis in 2019.
PMID: 31164994
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2013.
PMID: 23691025
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal Int J Mol Sci in 2019.
PMID: 31744099
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal Int J Mol Sci in 2019.
PMID: 31744099
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Oncogenesis in 2016.
PMID: 27918551
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Oncogenesis in 2016.
PMID: 27918551
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2013.
PMID: 23691025
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS Biol in 2018.
PMID: 29337987
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS Biol in 2018.
PMID: 29337987
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal Int J Mol Sci in 2019.
PMID: 31744099
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Oncogenesis in 2016.
PMID: 27918551
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Nat Commun in 2019.
PMID: 31409793
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal ScientificWorldJournal in 2014.
PMID: 24578622
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal Int J Mol Sci in 2017.
PMID: 28926934
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2016.
PMID: 27281159
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2016.
PMID: 27404495
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal PLoS Genet in 2015.
PMID: 25760041
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Sci Rep in 2017.
PMID: 28325912
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2017.
PMID: 29016672
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal J Transl Med in 2017.
PMID: 28472954
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal J Transl Med in 2017.
PMID: 28472954
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal J Transl Med in 2017.
PMID: 28472954
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2015.
PMID: 25815818
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2015.
PMID: 25815818
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal J Transl Med in 2017.
PMID: 28472954
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2015.
PMID: 25815818
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2015.
PMID: 25815818
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2015.
PMID: 26161782
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Sci Rep in 2016.
PMID: 26838546
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Cell Death Dis in 2013.
PMID: 23788038
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Cell Death Dis in 2013.
PMID: 23788038
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Cell Death Dis in 2013.
PMID: 23788038
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Cell Death Dis in 2013.
PMID: 23788038
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal Int J Mol Sci in 2017.
PMID: 28837077
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Pig
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2014.
PMID: 24533157
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Biosci Rep in 2018.
PMID: 29789400
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Oncotarget in 2015.
PMID: 25869099
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Cancer Med in 2019.
PMID: 31274246
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Sci Rep in 2018.
PMID: 29968775
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Sci Rep in 2018.
PMID: 29968775
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Oncogenesis in 2015.
PMID: 26690546
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu, Rat
Figure Note :
The data was peer reviewed and published in the journal J Cancer in 2019.
PMID: 31762816
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu, Ms
Figure Note :
The data was peer reviewed and published in the journal Oncotarget in 2015.
PMID: 25869099
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Int J Mol Sci in 2019.
PMID: 31323961
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Int J Mol Sci in 2019.
PMID: 31323961
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Int J Mol Sci in 2019.
PMID: 31323961
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Int J Mol Sci in 2019.
PMID: 31323961
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Materials (Basel) in 2018.
PMID: 30223515
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2014.
PMID: 25437003
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Oncotarget in 2016.
PMID: 27036017
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Oncotarget in 2016.
PMID: 27036017
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Oncotarget in 2016.
PMID: 27036017
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Oncotarget in 2016.
PMID: 27036017
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Oncotarget in 2016.
PMID: 27036017
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Oncotarget in 2016.
PMID: 27036017
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Oncotarget in 2016.
PMID: 27036017
Date :
Beished Shabangu submitted on 17-Nov-2019
Application Tested :
WB
Sample Species :
Ms
Sample :
N2a cells
Blocking :
5% Milk, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 1Hr
Date :
Anonymous submitted on 29-Oct-2019
Application Tested :
WB
Sample Species :
Sheep
Sample :
Endometrium extracts
Amount used :
30 μg
Blocking :
Block Ace (KAC Co., Ltd.), 25°C, 1Hr
Primary Antibody :
1:5000, 4°C, 16Hr
Date :
Anonymous submitted on 17-Dec-2015
Application Tested :
WB
Sample Species :
Cat
Sample :
Mya-1 cells
Amount used :
60μg
Blocking :
5% milk, 22°C, 1Hr
Primary Antibody :
1:5000, 22°C, 1Hr
Date :
Anonymous submitted on 24-Jul-2015
Application Tested :
WB
Sample Species :
Hu
Sample :
Human Periodontal ligament cells
Amount used :
10μg
Blocking :
ECL Prime Blocking Reagent, 25°C, 0.5Hr
Primary Antibody :
1:5000, 25°C, 1Hr
Date :
Anonymous submitted on 17-Jul-2015
Application Tested :
WB
Sample Species :
Ms
Sample :
ileum tissue, heart tissue, skeletal muscle tissue, brain tissue
Amount used :
50-100 μg
Blocking :
5% skim milk in PBS, 25°C, 1Hr
Primary Antibody :
1:2000, 37°C, 0.5Hr
Lane Description :
Lane 1: 100ug mouse ileum tissue, Lane 2: 50ug mouse heart tissue, Lane 3: 100ug mouse skeletal muscle tissue, Lane 4: 50ug mouse brain tissue
Date :
Anonymous submitted on 13-Feb-2015
Application Tested :
WB
Sample Species :
Hu
Sample :
HeLa cells, HCT116 cells
Amount used :
50μg
Blocking :
1% milk in PBST, 25°C, 1Hr
Primary Antibody :
1:10000, 4°C, 14Hr
Lane Description :
Lane1:HeLa cells Lane2:HCT116 cells
Date :
Anonymous submitted on 09-Jan-2015
Application Tested :
WB
Sample Species :
Ms
Sample :
OKT3 cells
Amount used :
50μg
Blocking :
5% skim milk, 25°C, 1Hr
Primary Antibody :
1:10000, 25°C, 1Hr
Date :
Anonymous submitted on 07-Jan-2015
Application Tested :
WB
Sample Species :
Rat
Sample :
H9C2 cells
Amount used :
30μg
Blocking :
5% non-fat milk, 25°C, 1Hr
Primary Antibody :
1:5000, 25°C, 1Hr
Date :
Anonymous submitted on 23-Dec-2013
Application Tested :
WB
Sample Species :
Ms
Sample :
mouse aorta tissue
Amount used :
4μg
Blocking :
3% milk in PBST, 23°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Date :
Anonymous submitted on 22-Dec-2011
Application Tested :
WB
Sample Species :
Yeast
Sample :
Yeast wild type 303
Blocking :
5% milk in TBST, 25°C, 2Hr
Primary Antibody :
1:1000, 25°C, 2Hr
Date :
Anonymous submitted on 21-Oct-2011
Application Tested :
WB
Sample Species :
Rice
Sample :
Rice leaf
Amount used :
30μg
Blocking :
5% skim milk in PBST, 25°C, 1Hr
Primary Antibody :
1:1000, 25°C, 1Hr