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PDE10A antibody [C2C3], C-term

Comparison Overexpression
Anti-PDE10A antibody [C2C3], C-term used in Western Blot (WB). GTX118886
Anti-PDE10A antibody [C2C3], C-term used in Western Blot (WB). GTX118886
Anti-PDE10A antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118886
Anti-PDE10A antibody [C2C3], C-term used in Western Blot (WB). GTX118886
Anti-PDE10A antibody [C2C3], C-term used in Western Blot (WB). GTX118886
Anti-PDE10A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX118886

Cat. No. GTX118886

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PDE10A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

Myc-DDK-tagged PDE10A-transfected 293T

Predict Reactivity

Zebrafish(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PDE10A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618100

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphodiesterase 10A , ADSD2 , HSPDE10A , IOLOD , LINC00473 , PDE10A19

Cellular Localization

Cytoplasm

Background

Various cellular responses are regulated by the second messengers cAMP and cGMP. Phosphodiesterases, such as PDE10A, eliminate cAMP- and cGMP-mediated intracellular signaling by hydrolyzing the cyclic nucleotide to the corresponding nucleoside 5-prime monophosphate (Fujishige et al., 2000 [PubMed 10998054]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PDE10A antibody [C2C3], C-term used in Western Blot (WB). GTX118886

GTX118886 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDE10A antibody [C2C3], C-term (GTX118886) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PDE10A antibody [C2C3], C-term used in Western Blot (WB). GTX118886

GTX118886 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDE10A antibody [C2C3], C-term (GTX118886) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PDE10A antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118886

GTX118886 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PDE10A(GTX118886) antibody at 1:200 dilution.

Anti-PDE10A antibody [C2C3], C-term used in Western Blot (WB). GTX118886

GTX118886 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDE10A antibody [C2C3], C-term (GTX118886) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PDE10A antibody [C2C3], C-term used in Western Blot (WB). GTX118886

GTX118886 WB Image

Comparison

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with PDE10A antibody [C2C3], C-term (GTX118886) diluted at 1:1000 and competitor's antibody (ab177933) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-PDE10A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX118886

GTX118886 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PDE10A(GTX118886) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Kevin J Lee et al. Cancer Prev Res (Phila) 2021; 14 (11) : 995-1008 Suppression of Colon Tumorigenesis in Mutant Apc Mice by a Novel PDE10 Inhibitor that Reduces Oncogenic β-Catenin.
Application : WB
Reactivity : Mouse
Fusco JP et al. Cancer Med 2018; (Epub) Genomic characterization of individuals presenting extreme phenotypes of high and low risk to develop tobacco-induced lung cancer.
Application : IHC-P
Reactivity : Human
Li N et al. Oncogene 2015; 34 (12) : 1499-509 Phosphodiesterase 10A: a novel target for selective inhibition of colon tumor cell growth and β-catenin-dependent TCF transcriptional activity.
Application : WB, IHC-P
Reactivity : Human
Li N et al. Oncotarget 2015; 6 (29) : 27403-15 Suppression of β-catenin/TCF transcriptional activity and colon tumor cell growth by dual inhibition of PDE5 and 10.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169