Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
Clone CM1 , M9E7, M8C9 bind with high efficiency with different regions of GVTSAPDTRPAPGSTAPPAHGVTSA synthetic peptide spanning the one repeat of VNTR extracellular portion of MUC1 molecule. These antibodies react with VNTR5 and VNTR20 recombinant unglycosylated fragments of MUC1 protein and underglycosylated MUC1 prepared from tumor fluids (T47D cells) or as a result of chemical treatment (NaJO4) of human milk MUC1. They do not recognize natural MUC1 protein isolated from human milk by affinity purification on carbohydrate epitope specific antibodies and belong to monoclonal antibody cluster 1-4 according to ISOMB TD-4 classification. No cross-reactivity with egg white avidin.
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant (E. coli) VNTR20 (tandem repeat in MUC1 core) polypeptide.This antibody binds efficiently with different regions of GVTSAPDTRPAPGSTAPPAHGVTSA synthetic peptide spanning the one repeat of VNTR extracellular portion of MUC1 molecule.
Purification
Protein A purified
Conjugation
Unconjugated
RRID
AB_380883
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
mucin 1, cell surface associated , ADMCKD , ADMCKD1 , CA 15-3 , CD227 , EMA , H23AG , KL-6 , MAM6 , MCD , MCKD , MCKD1 , MUC-1 , MUC-1/SEC , MUC-1/X , MUC1/ZD , PEM , PEMT , PUM
Cellular Localization
Apical cell membrane , Cytoplasm , Nucleus
Background
This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]
Database
Research Area
