GeneTex

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United States (US)

IL6 antibody

Cat. No. GTX27737

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA, Neutralizing/Inhibition

Reactivity

 Mouse
Package
500 μl ($399)
See all IL6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.2μg/ml
ELISA 0.5-2μg/ml
Neutralizing/Inhibition Assay dependent

Note :

Neutralizing/Inhibition
Rabbit anti Mouse Interleukin-6 antibody neutralizes murine IL-6 activity with an ND50 for mIL-6 (3 pgml-1 of between 0.24 and 0.36μg ml-1.
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Product Note

This IL-6 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with GTX74037 as the detection reagent.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant murine IL-6

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_368318

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 6 , Il-6

Cellular Localization

Secreted

Background

This gene encodes a member of the interleukin family of cytokines that have important functions in immune response, hematopoiesis, inflammation and the acute phase response. The ectopic overexpression of the encoded protein in mice results in excessive plasma cells in circulation, leading to death. Mice lacking the encoded protein exhibit abnormalities in hepatic acute phase response, some immune mechanisms, bone resorption in response to estrogen, liver regeneration and wound healing. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Database

Research Area

REFERENCE

Application Reference

Ham DJ et al. Am J Physiol Endocrinol Metab 2016; 310 (11) : E970-81 Glycine restores the anabolic response to leucine in a mouse model of acute inflammation.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Package List Price ($)
$ 399