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Heavy chain cardiac Myosin antibody [3-48]

Cat. No. GTX20015

Reference 16

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3-48

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-Fr, ELISA

Reactivity

 Human, Mouse, Rat, Rabbit, Bovine, Dog, Pig
Package
100 μg ($399)
See all Heavy chain cardiac Myosin products

APPLICATION

Application Note

For ELISA: Use at an assay dependant dilution. For IF: Use at an assay dependant dilution. For IHC-Fr: Use at an assay dependant dilution. For WB: Use at an assay dependant dilution. Predicted molecular weight: 223 kDa. Optimal dilutions/concentrations should be determined by the researcher.

Positive Control

Slow skeletal muscle, fast skeletal muscle, eye muscle

Product Note

This antibody is myosin alpha and beta heavy chain specific. Affinity constants for:Human ventricular myosin heavy chains - 3.33 x 108M-1Human atrial myosin heavy chains - 1.48 x 108M-1 Human skeletal muscle myosin - 1.06 x 108M-1 Canine cardiac myosin heavy chains - 2.06 x 108M-1 3-48 antibody cross-reacts with heavy chains of human myosin isolated from: slow skeletal muscle, fast skeletal muscle, eye muscle, and streptococcal M5 peptide. It does not react with human platelet, smooth muscle myosin heavy chains, myosin light chains, myosin fragments circulating in blood of patients with myocardial infarction, nor does it react with any other human tissue antigens or blood cells.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length native protein (purified) (Human).

Purification

IgG fraction

Conjugation

Unconjugated

RRID

AB_373882

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ASD3 , 160710 , Myosin cardiac Heavy chain , alphaMHC , alpha-MHC , 4624 , MYHCA , P13533 , MYH6 , MYHC , alpha MHC

Cellular Localization

Thick filaments of the myofibrils

Background

3-48 antibody cross-reacts with heavy chains of human myosin isolated from: slow skeletal muscle, fast skeletal muscle, eye muscle, and streptococcal M5 peptide. It does not react with human platelet, smooth muscle myosin heavy chains, myosin light chains, myosin fragments circulating in blood of patients with myocardial infarction, nor does it react with any other human tissue antigens or blood cells.

Research Area

REFERENCE

Application Reference

Wang J et al. Epigenetics 2022; 17 (12) : 1686-1700 CircRIMKLB promotes myoblast proliferation and inhibits differentiation by sponging miR-29c to release KCNJ12.
Application : WB, ICC/IF
Reactivity : Mouse
Chen M et al. Epigenetics 2022; : 1-22 CircCLTH promotes skeletal muscle development and regeneration
Application : WB, ICC/IF
Reactivity : Bovine
Ibrahim Elsaeid Elnour et al. Cells 2021; 10 (1) Circular RNA circMYL1 Inhibit Proliferation and Promote Differentiation of Myoblasts by Sponging miR-2400.
Application : ICC/IF
Reactivity : Bovine
Boimpoundi Eunice Flavie Ouali et al. Biochem Biophys Rep 2021; 26 : 101019 Beta-agonist drugs modulate the proliferation and differentiation of skeletal muscle cells in vitro.
Application : WB
Reactivity : Mouse
Ru W et al. J Anim Sci Biotechnol 2021; 12 (1) : 102 The circular RNA circCPE regulates myoblast development by sponging miR-138.
Application : ICC/IF
Reactivity : Mouse
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REVIEW

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Application Reference
Wang J et al. Epigenetics 2022; 17 (12):1686-1700 CircRIMKLB promotes myoblast proliferation and inhibits differentiation by sponging miR-29c to release KCNJ12.
Application : WB, ICC/IF
Reactivity : Mouse
Chen M et al. Epigenetics 2022;:1-22 CircCLTH promotes skeletal muscle development and regeneration
Application : WB, ICC/IF
Reactivity : Bovine
Ibrahim Elsaeid Elnour et al. Cells 2021; 10 (1) Circular RNA circMYL1 Inhibit Proliferation and Promote Differentiation of Myoblasts by Sponging miR-2400.
Application : ICC/IF
Reactivity : Bovine
Boimpoundi Eunice Flavie Ouali et al. Biochem Biophys Rep 2021; 26:101019 Beta-agonist drugs modulate the proliferation and differentiation of skeletal muscle cells in vitro.
Application : WB
Reactivity : Mouse
Ru W et al. J Anim Sci Biotechnol 2021; 12 (1):102 The circular RNA circCPE regulates myoblast development by sponging miR-138.
Application : ICC/IF
Reactivity : Mouse
Xuemei Shen et al. Front Cell Dev Biol 2020; 8:615638 CircINSR Regulates Fetal Bovine Muscle and Fat Development.
Application : ICC/IF
Reactivity : Human
Poller W et al. J Am Heart Assoc 2020; 9 (10):e015289 Familial Recurrent Myocarditis Triggered by Exercise in Patients With a Truncating Variant of the Desmoplakin Gene.
Application : ELISA
Elsaeid Elnour I et al. J Cell Physiol 2020; (Epub) Bta-miR-885 promotes proliferation and inhibits differentiation of myoblasts by targeting MyoD1.
Wang X et al. Mol Ther Nucleic Acids 2019; 18:966-980 Circular RNA TTN Acts As a miR-432 Sponge to Facilitate Proliferation and Differentiation of Myoblasts via the IGF2/PI3K/AKT Signaling Pathway.
Xiaogang Wang et al. Molecular Therapy: Nucleic Acid 2019; (Epub) Circular RNA TTN acts as a miR-432 sponge to facilitate proliferation and differentiation of myoblasts via the IGF2/PI3K/AKT signaling pathway.
Application : WB, ICC/IF
Reactivity : Bovine
Kyndiah A et al. Biosens Bioelectron 2019;:111844 Bioelectronic Recordings of Cardiomyocytes with Accumulation Mode Electrolyte Gated Organic Field Effect Transistors.
Application : ICC/IF
Reactivity : Human
HeidrunSteinle et al. Molecular Therapy Nucleic Acids 2019; (Epub) Reprogramming of urine-derived renal epithelial cells into iPSCs using self-replicating RNA and consecutive differentiation into beating cardiomyocytes.
Application : ICC/IF
Reactivity : Human
Garreta E et al. Biomaterials 2016; 98:64-78 Myocardial commitment from human pluripotent stem cells: Rapid production of human heart grafts.
Application : ICC/IF
Reactivity : Human
Nguyen NU et al. PLoS One 2015; 10 (4):e0124762 Dual roles of palladin protein in in vitro myogenesis: inhibition of early induction but promotion of myotube maturation.
Application : WB
Reactivity : Mouse
Connell JP et al. J Cell Mol Med 2013; 17 (6):774-81 Formation of functional gap junctions in amniotic fluid-derived stem cells induced by transmembrane co-culture with neonatal rat cardiomyocytes.
Application : ICC/IF
Reactivity : Rat
Awasthi S et al. J Biophotonics 2012; 5 (1):57-66 Label-free identification and characterization of human pluripotent stem cell-derived cardiomyocytes using second harmonic generation (SHG) microscopy.
Package List Price ($)
$ 399
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