GRP94 antibody [N1N3]

Name: GRP94 antibody [N1N3]
Brand: GeneTex
SKU: GTX103203
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-GRP94 antibody [N1N3] used in Western Blot (WB). GTX103203

Anti-GRP94 antibody [N1N3] used in Immunoprecipitation (IP). GTX103203

Anti-GRP94 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103203

Anti-GRP94 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103203

Cat. No. GTX103203

Reference 8

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all GRP94 products

View product citations for antibody GTX103203 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

NIH-3T3 , H1299 , HeLaS3 , 293T

Predict Reactivity

Rat, Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GRP94. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950517

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein 90 beta family member 1 , ECGP , GP96 , GRP94 , HEL-S-125m , HEL35 , TRA1

Cellular Localization

Endoplasmic reticulum lumen , Melanosome

Background

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]

Database

Gene ID: 7184 HSP90B1
UniProt: P14625 HSP90B1

Research Area

DATA IMAGES

GTX103203 WB Image

Wild-type (WT) and GRP94 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP94 antibody [N1N3] (GTX103203) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103203 IP Image

Immunoprecipitation of GRP94 protein from HeLa whole cell extracts using 5 μg of GRP94 antibody [N1N3] (GTX103203).
Western blot analysis was performed using GRP94 antibody [N1N3] (GTX103203).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX103203 ICC/IF Image

GRP94 antibody [N1N3] detects GRP94 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GRP94 protein stained by GRP94 antibody [N1N3] (GTX103203) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX103203 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela S3
7.5% SDS PAGE
GTX103203 diluted at 1:1000

GTX103203 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCM-1 xenograft , using HSP90B(GTX103203) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min