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DDX58 antibody

Anti-DDX58 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85488
Anti-DDX58 antibody used in IHC (Paraffin sections) (IHC-P). GTX85488
Anti-DDX58 antibody used in Western Blot (WB). GTX85488
Anti-DDX58 antibody used in IHC (Paraffin sections) (IHC-P). GTX85488
Anti-DDX58 antibody used in IHC (Paraffin sections) (IHC-P). GTX85488
Anti-DDX58 antibody used in IHC (Paraffin sections) (IHC-P). GTX85488
Anti-DDX58 antibody used in Western Blot (WB). GTX85488
Anti-DDX58 antibody used in IHC (Paraffin sections) (IHC-P). GTX85488
Anti-DDX58 antibody used in IHC (Paraffin sections) (IHC-P). GTX85488

Cat. No. GTX85488

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all DDX58 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 2 μg/mL
ICC/IF Assay dependent
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

107 kDa. ( Note )

Positive Control

C2C12 Cell Lysate(GTX27182) , Human Heart Tissue Lysate(GTX27916) , Human Heart Tissue Slide

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

DDX58 antibody was raised against human GST-tagged DDX58 protein.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_10724957

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DExD/H-box helicase 58 , RIG-I , RIG1 , RIGI , RLR-1 , SGMRT2

Cellular Localization

Cytoplasm,Cytoplasm, cytoskeleton

Background

The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the cytoplasmic helicase retinoic acid inducible gene protein 1 (RIG-1) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. Unlike TLR3 however, RIG-1 activates the kinases TBK1 and IKKepsilon through the adaptor protein IPS-1. These kinases then phosphorylate the transcription factors IRF-3 and IRF-7 which are essential for the expression of type-I interferons. RIG-1 is required for the production of interferons in response to RNA viruses including paramyxoviruses, influenza virus, and Japanese encephalitis virus.

Database

Research Area

DATA IMAGES

Anti-DDX58 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85488

GTX85488 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX85488 DDX58 antibody.
Dilution : 20 μg/ml

Anti-DDX58 antibody used in IHC (Paraffin sections) (IHC-P). GTX85488

GTX85488 IHC-P Image

IHC-P analysis of human testis tissue using GTX85488 DDX58 antibody.
Dilution : 20 μg/ml

Anti-DDX58 antibody used in Western Blot (WB). GTX85488

GTX85488 WB Image

WB analysis of C2C12 cell lysate using GTX85488 DDX58 antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml

Anti-DDX58 antibody used in IHC (Paraffin sections) (IHC-P). GTX85488

GTX85488 IHC-P Image

IHC-P analysis of human heart tissue using GTX85488 DDX58 antibody.
Working concentration : 5 μg/ml

Anti-DDX58 antibody used in IHC (Paraffin sections) (IHC-P). GTX85488

GTX85488 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX85488 DDX58 antibody.
Dilution : 20 μg/ml

Anti-DDX58 antibody used in IHC (Paraffin sections) (IHC-P). GTX85488

GTX85488 IHC-P Image

IHC-P analysis of rat heart tissue using GTX85488 DDX58 antibody.
Dilution : 10 μg/ml

Anti-DDX58 antibody used in Western Blot (WB). GTX85488

GTX85488 WB Image

WB analysis of A20 cell (Lane 1) and rat heart tissue (Lane 2) lysate using GTX85488 DDX58 antibody.
Dilution : 2 μg/ml

Anti-DDX58 antibody used in IHC (Paraffin sections) (IHC-P). GTX85488

GTX85488 IHC-P Image

IHC-P analysis of rat testis tissue using GTX85488 DDX58 antibody.
Dilution : 20 μg/ml

Anti-DDX58 antibody used in IHC (Paraffin sections) (IHC-P). GTX85488

GTX85488 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX85488 DDX58 antibody.
Dilution : 1 μg/ml

REFERENCE

Application Reference

Te Velthuis AJW et al. Nat Microbiol 2018; 3 (11) : 1234-1242 Mini viral RNAs act as innate immune agonists during influenza virus infection.
Aartjan te Velthuis et al. bioRxiv 2018; (Epub) Mini viral RNAs act as innate immune agonists during influenza virus infection.
Application : WB
Reactivity : Human
Chang YC et al. J Hematol Oncol 2017; 10 (1) : 11 Glucose transporter 4 promotes head and neck squamous cell carcinoma metastasis through the TRIM24-DDX58 axis.
Application : WB
Reactivity : Human
Chen N et al. Microb Pathog 2016; 100 : 84-89 enterovirus 71 inhibits cellular type i * signaling by inhibiting host rig-i ubiquitination.
Application : WB
Reactivity : Human
Xing J et al. J Virol 2012; 86 (7) : 3528-40 Herpes simplex virus 1 tegument protein US11 downmodulates the RLR signaling pathway via direct interaction with RIG-I and MDA-5.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399