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Wnt5a antibody

Overexpression
Anti-Wnt5a antibody used in Western Blot (WB). GTX111187
Anti-Wnt5a antibody used in Western Blot (WB). GTX111187
Anti-Wnt5a antibody used in Western Blot (WB). GTX111187
Anti-Wnt5a antibody used in IHC (Paraffin sections) (IHC-P). GTX111187
Anti-Wnt5a antibody used in IHC (Paraffin sections) (IHC-P). GTX111187

Cat. No. GTX111187

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Wnt5a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

rat heart

Predict Reactivity

Rabbit, Bovine, Dog, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.09 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WNT5A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617364

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Wnt family member 5A , hWNT5A

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Wnt5a antibody used in Western Blot (WB). GTX111187

GTX111187 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt5a antibody (GTX111187) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Wnt5a antibody used in Western Blot (WB). GTX111187

GTX111187 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt5a antibody (GTX111187) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Wnt5a antibody used in Western Blot (WB). GTX111187

GTX111187 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with WNT5A antibody (GTX111187) diluted at 1:500.

Anti-Wnt5a antibody used in IHC (Paraffin sections) (IHC-P). GTX111187

GTX111187 IHC-P Image

Wnt5a antibody detects secreted Wnt5a protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Wnt5a stained by Wnt5a antibody (GTX111187) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Wnt5a antibody used in IHC (Paraffin sections) (IHC-P). GTX111187

GTX111187 IHC-P Image

Wnt5a antibody detects secreted Wnt5a protein by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
Wnt5a stained by Wnt5a antibody (GTX111187) diluted at 1:500.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

REFERENCE

Application Reference

Yan Zou et al. Cell Death Dis 2021; 12 (10) : 877 Cardiac Wnt5a and Wnt11 promote fibrosis by the crosstalk of FZD5 and EGFR signaling under pressure overload.
Application : WB
Reactivity : Human, Mouse
Guo CH et al. Mar Drugs 2021; 19 (4) Combination of Fish Oil and Selenium Enhances Anticancer Efficacy and Targets Multiple Signaling Pathways in Anti-VEGF Agent Treated-TNBC Tumor-Bearing Mice.
Application : WB
Reactivity : Mouse
Shen HH et al. J Biomed Sci 2019; 26 (1) : 62 Raloxifene inhibits adipose tissue inflammation and adipogenesis through Wnt regulation in ovariectomized rats and 3?3-L1 cells.
Application : WB
Reactivity : Rat
Gong W et al. J Cell Biochem 2018; (Epub) Deletion of long noncoding RNA EFNA3 aggravates hypoxia-induced injury in PC-12 cells by upregulation of miR-101a.
Application : WB
Reactivity : Rat
Lin HK et al. J Cell Mol Med 2018; (Epub) Caveolin-1 down-regulation is required for Wnt5a-Frizzled 2 signalling in Ha-RasV12 -induced cell transformation.
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REVIEW

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Application Reference
Yan Zou et al. Cell Death Dis 2021; 12 (10):877 Cardiac Wnt5a and Wnt11 promote fibrosis by the crosstalk of FZD5 and EGFR signaling under pressure overload.
Application : WB
Reactivity : Human, Mouse
Guo CH et al. Mar Drugs 2021; 19 (4) Combination of Fish Oil and Selenium Enhances Anticancer Efficacy and Targets Multiple Signaling Pathways in Anti-VEGF Agent Treated-TNBC Tumor-Bearing Mice.
Application : WB
Reactivity : Mouse
Shen HH et al. J Biomed Sci 2019; 26 (1):62 Raloxifene inhibits adipose tissue inflammation and adipogenesis through Wnt regulation in ovariectomized rats and 3?3-L1 cells.
Application : WB
Reactivity : Rat
Gong W et al. J Cell Biochem 2018; (Epub) Deletion of long noncoding RNA EFNA3 aggravates hypoxia-induced injury in PC-12 cells by upregulation of miR-101a.
Application : WB
Reactivity : Rat
Lin HK et al. J Cell Mol Med 2018; (Epub) Caveolin-1 down-regulation is required for Wnt5a-Frizzled 2 signalling in Ha-RasV12 -induced cell transformation.
Wang LT et al. Oncogene 2017; (Epub) Transcription factor SPZ1 promotes TWIST-mediated epithelial-mesenchymal transition and oncogenesis in human liver cancer.
Application : WB
Reactivity : Human
Huang T et al. Nat Commun 2016; 7:12885 A regulatory circuit of miR-125b/miR-20b and Wnt signalling controls glioblastoma phenotypes through FZD6-modulated pathways.
Borcherding N et al. Cancer Res 2015; 75 (10):1972-82 Paracrine WNT5A Signaling Inhibits Expansion of Tumor-Initiating Cells.
Application : WB, IHC-P
Reactivity : Mouse
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169