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DNA ligase IV antibody [N2C2], Internal

KO/KD
Anti-DNA ligase IV antibody [N2C2], Internal used in Western Blot (WB). GTX100100
Anti-DNA ligase IV antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100100

Cat. No. GTX100100

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all DNA ligase IV products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Positive Control

Molt-4

Predict Reactivity

Rat, Sheep, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DNA ligase IV. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950812

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA ligase 4 , LIG4S

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DNA ligase IV antibody [N2C2], Internal used in Western Blot (WB). GTX100100

GTX100100 WB Image

Sample(30 μg of whole cell lysate)
A:MOLT4(GTX27912) 7.5% SDS PAGE
GTX100100 diluted at 1:1000

Anti-DNA ligase IV antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100100

GTX100100 IHC-P Image

DNA ligase IV antibody [N2C2], Internal detects DNA ligase IV protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
DNA ligase IV stained by DNA ligase IV antibody [N2C2], Internal (GTX100100) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Trivedi P et al. Nature 2023; 618 (7967) : 1049-1056 Mitotic tethering enables inheritance of shattered micronuclear chromosomes.
Britton S et al. Nucleic Acids Res 2020; 48 (17) : 9710-9723 ATM antagonizes NHEJ proteins assembly and DNA-ends synapsis at single-ended DNA double strand breaks.
Application : WB
Reactivity : Human
Zhu S et al. Nucleic Acids Res 2017; 45 (18) : 10583-10594 Protein phosphatase 1 and phosphatase 1 nuclear targeting subunit-dependent regulation of DNA-dependent protein kinase and non-homologous end joining.
Ly P et al. Nat Cell Biol 2016; Selective Y centromere inactivation triggers chromosome shattering in micronuclei and repair by non-homologous end joining.
Application : WB
Reactivity : Human
You BJ et al. Food Chem Toxicol 2014; 65 : 205-12 Non-homologous end joining pathway is the major route of protection against 4β-hydroxywithanolide E-induced DNA damage in MCF-7 cells.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169