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Biotin antibody

Anti-Biotin antibody used in Western Blot (WB). GTX124262

Cat. No. GTX124262

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all Biotin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:90000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to Biotin

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174491

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Biotin antibody used in Western Blot (WB). GTX124262

GTX124262 WB Image

Western blot experiment using Biotin Antibody (GTX124262) at 1:90000 dilution to detect 60ng of biotinylated protein and non-biotinylated control.
A: Non-biotinlated control.
B: Biotinylated protein.

REFERENCE

Application Reference

Wang SA et al. Cell Death Differ 2021; (Epub) USP24 promotes drug resistance during cancer therapy.
Application : WB
Heit C et al. Free Radic Biol Med 2016; Catalase Deletion Promotes PreDiabetic Phenotype in Mice.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169