ORP150 antibody [C2C3], C-term

Name: ORP150 antibody [C2C3], C-term
Brand: GeneTex
SKU: GTX102255
Price: 399.00 USD

Anti-ORP150 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102255

Anti-ORP150 antibody [C2C3], C-term used in Western Blot (WB). GTX102255

Anti-ORP150 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102255

Cat. No. GTX102255

Reference 9

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat, Plant
Package	100 μl ($399), 25 μl ($169)

See all ORP150 products

View product citations for antibody GTX102255 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

111 kDa. ( Note )

Positive Control

mouse liver , rat brain , MCF-7 , MDA-MB-231

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ORP150. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950534

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hypoxia up-regulated 1 , GRP-170 , Grp170 , HSP12A , IMD59 , ORP-150 , ORP150

Cellular Localization

Endoplasmic reticulum lumen

Background

The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal. [provided by RefSeq]

Database

Gene ID: 10525 HYOU1
UniProt: Q9Y4L1 HYOU1

Research Area

- Cancer
- Cancer
- Hypoxia

DATA IMAGES

GTX102255 ICC/IF Image

ORP150 antibody [C2C3], C-term detects ORP150 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ORP150 protein stained by ORP150 antibody [C2C3], C-term (GTX102255) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX102255 WB Image

ORP150 antibody detects ORP150 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 5% SDS-PAGE, and blotted with ORP150 antibody (GTX102255) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102255 IHC-P Image

ORP150 antibody [C2C3], C-term detects ORP150 protein at cytoplasm on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
ORP150 antibody [C2C3], C-term (GTX102255) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102255 ICC/IF Image

ORP150 antibody [C2C3], C-term detects ORP150 protein at cytoplasm by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ORP150 protein stained by ORP150 antibody [C2C3], C-term (GTX102255) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX102255 WB Image

ORP150 antibody detects ORP150 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 5% SDS-PAGE, and blotted with ORP150 antibody (GTX102255) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102255 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep3B, using ORP150(GTX102255) antibody at 1:500 dilution.

GTX102255 WB Image

ORP150 antibody detects ORP150 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and blotted with ORP150 antibody (GTX102255) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102255 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using ORP150(GTX102255) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min