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ATG7 antibody [N3C2], Internal

Anti-ATG7 antibody [N3C2], Internal used in Western Blot (WB). GTX113613

Cat. No. GTX113613

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ATG7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATG7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036291

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy related 7 , APG7-LIKE , APG7L , GSA7

Cellular Localization

Cytoplasm

Background

This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ATG7 antibody [N3C2], Internal used in Western Blot (WB). GTX113613

GTX113613 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG7 antibody [N3C2], Internal (GTX113613) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Palanisamy K et al. J Cell Physiol 2018; (Epub) RNA-binding protein, human antigen R regulates hypoxia-induced autophagy by targeting ATG7/ATG16L1 expressions and autophagosome formation.
Application : WB
Reactivity : Human
Chang HP et al. Int J Oncol 2018; (Epub) pterostilbene modulates the suppression of multidrug resistance protein-1 and triggers autophagic and apoptotic mechanisms in *-resistant human oral cancer car cells via akt signaling.
Application : WB
Reactivity : Human
Bechor S et al. Biochim Biophys Acta 2017; 1862 (9) : 1001-1012 Adipose tissue conditioned media support macrophage lipid-droplet biogenesis by interfering with autophagic flux.
Application : WB
Reactivity : Mouse
Chi HC et al. Autophagy 2016; : 43115 Thyroid hormone suppresses hepatocarcinogenesis via DAPK2 and SQSTM1-dependent selective autophagy.
Application : WB
Reactivity : Mouse
Matsuzawa T et al. Immunology 2014; 141 (1) : 22525 autophagy activation by *-γ via the p38 mitogen-activated protein kinase signalling pathway is involved in macrophage bactericidal activity.
Application : WB
Reactivity : Mouse
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169