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MIA3 antibody [N1], N-term

Anti-MIA3 antibody [N1], N-term used in Western Blot (WB). GTX101827
Anti-MIA3 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101827
Anti-MIA3 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX101827

Cat. No. GTX101827

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MIA3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 10 μg/ml
Not tested in other applications.

Calculated MW

214 kDa. ( Note )

Positive Control

A549 , HepG2

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human MIA3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240479

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MIA SH3 domain ER export factor 3 , ARNT , D320 , TANGO , TANGO1 , UNQ6077

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type I membrane protein

Background

Required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. May participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1.

Database

Research Area

DATA IMAGES

Anti-MIA3 antibody [N1], N-term used in Western Blot (WB). GTX101827

GTX101827 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: Hep G2 (GTX27900)
5% SDS PAGE
GTX101827 diluted at 1:1000

Anti-MIA3 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101827

GTX101827 ICC/IF Image

MIA3 antibody [N1], N-term detects MIA3 protein at Cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in -20ºC 100% MeOH for 5 min.
Green: MIA3 protein stained by MIA3 antibody [N1], N-term (GTX101827) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-MIA3 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX101827

GTX101827 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human testis, using MIA3(GTX101827) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Gao H et al. Cancer Cell Int 2017; 17 : 78 MicroRNA-222 influences migration and invasion through MIA3 in colorectal cancer.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169