Nanog antibody [N3C3]

Name: Nanog antibody [N3C3]
Brand: GeneTex
SKU: GTX100863
Price: 399.00 USD

Anti-Nanog antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100863

Anti-Nanog antibody [N3C3] used in Western Blot (WB). GTX100863

Anti-Nanog antibody [N3C3] used in Immunoprecipitation (IP). GTX100863

Anti-Nanog antibody [N3C3] used in ELISA (ELISA). GTX100863

Cat. No. GTX100863

Reference 34

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, IHC, Sandwich ELISA
Reactivity	Human, Mouse, Ceratotherium simum
Package	100 μl ($399), 25 μl ($169)

See all Nanog products

View product citations for antibody GTX100863 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	Assay dependent
IHC-Fr	Assay dependent
IP	1:100-1:500
ELISA	1:1000-1:10000
IHC	Assay dependent
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture: GTX100863 , Detection: GTX89079

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

HeLa nucleus

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10615506

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Nanog homeobox

Cellular Localization

Nucleus

Background

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

Database

Gene ID: 79923 NANOG
UniProt: Q9H9S0 NANOG

Research Area

DATA IMAGES

GTX100863 ICC/IF Image

NANOG antibody [N3C3] detects NANOG protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min.
Green: NANOG protein stained by NANOG antibody [N3C3] (GTX100863) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 6 μm.

GTX100863 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa nucleus
12% SDS PAGE
GTX100863 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100863 IP Image

Immunoprecipitation of NANOG protein from NT2D1 whole cell extracts using 5 μg of NANOG antibody [N3C3] (GTX100863).
Western blot analysis was performed using NANOG antibody [N3C3] (GTX100863) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.