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B-Raf antibody [N2C1], Internal

Overexpression
Anti-B-Raf antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100913
Anti-B-Raf antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX100913
Anti-B-Raf antibody [N2C1], Internal used in Western Blot (WB). GTX100913
Anti-B-Raf antibody [N2C1], Internal used in Western Blot (WB). GTX100913
Anti-B-Raf antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100913
Anti-B-Raf antibody [N2C1], Internal used in Western Blot (WB). GTX100913

Cat. No. GTX100913

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all B-Raf products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

PC-12 , human BRAF-transfected 293T cells

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human B-Raf. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036363

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

B-Raf proto-oncogene, serine/threonine kinase , B-RAF1 , B-raf , BRAF1 , NS7 , RAFB1

Cellular Localization

Cytoplasm

Background

This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-B-Raf antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100913

GTX100913 IHC-P Image

B-Raf antibody [N2C1], Internal detects B-Raf protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
B-Raf antibody [N2C1], Internal (GTX100913) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-B-Raf antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX100913

GTX100913 IP Image

B-Raf antibody [N2C1], Internal immunoprecipitates B-Raf protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of B-Raf protein expressing HepG2 cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of B-Raf by 3 μg of B-Raf antibody [N2C1], Internal (GTX100913) 10% SDS-PAGE The immunoprecipitated B-Raf protein was detected by B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:500. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-B-Raf antibody [N2C1], Internal used in Western Blot (WB). GTX100913

GTX100913 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-B-Raf antibody [N2C1], Internal used in Western Blot (WB). GTX100913

GTX100913 WB Image

Overexpression

B-Raf antibody [N2C1], Internal detects B-Raf protein by western blot analysis. Non-transfected (-) and B-Raf-transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:5000.

Anti-B-Raf antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100913

GTX100913 IHC-P Image

B-Raf antibody [N2C1], Internal detects B-Raf protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
B-Raf stained by B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-B-Raf antibody [N2C1], Internal used in Western Blot (WB). GTX100913

GTX100913 WB Image

B-Raf antibody [N2C1], Internal detects B-Raf protein by western blot analysis.
A. 30 μg PC-12 whole cell extract
7.5 % SDS-PAGE
B-Raf antibody [N2C1], Internal (GTX100913) dilution: 1:5000

REFERENCE

Application Reference

Tsuji S et al. Oncol Lett 2021; 21 (2) : 113 Perphenazine exerts antitumor effects on HUT78 cells through Akt dephosphorylation by protein phosphatase 2A.
Application : WB
Reactivity : Human
Tateishi K et al. Acta Neuropathol Commun 2020; 8 (1) : 139 BRAF V600E mutation mediates FDG-methionine uptake mismatch in polymorphous low-grade neuroepithelial tumor of the young.
Application : WB
Reactivity : Human
Sase H et al. Mol Cancer Ther 2018; (Epub) Acquired JHDM1D-BRAF fusion confers resistance to FGFR inhibition in FGFR2- amplified gastric cancer.
Application : WB
Reactivity : Human
Rubin B et al. Cancer Invest 2015; (Epub) Mitogen-Activated Protein Kinase Pathway: Genetic Analysis of 95 Adrenocortical Tumors.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169