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Dengue virus NS3 protein antibody [GT2811]

Anti-Dengue virus NS3 protein antibody [GT2811] used in Western Blot (WB). GTX629477
Anti-Dengue virus NS3 protein antibody [GT2811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629477
Anti-Dengue virus NS3 protein antibody [GT2811] used in Western Blot (WB). GTX629477
Anti-Dengue virus NS3 protein antibody [GT2811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629477
Anti-Dengue virus NS3 protein antibody [GT2811] used in Western Blot (WB). GTX629477

Cat. No. GTX629477

Reference 17
3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT2811

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA

Reactivity

 Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4
Package
100 μl ($399),
25 μl ($169)
See all Dengue virus NS3 protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

Dengue virus 2 infected BHK21 cells , Dengue virus type 3 infected BHK-21 cells , Dengue virus 2-infected C6/36

Product Note

This antibody does not cross-react with JEV Envelope protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Dengue virus NS3 protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2801283

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Dengue Virus Non-structural protein 3 , Dengue Virus NS3 , DENV Non-structural protein 3 , DENV NS3

Research Area

DATA IMAGES

Anti-Dengue virus NS3 protein antibody [GT2811] used in Western Blot (WB). GTX629477

GTX629477 WB Image

NS3 (Dengue virus) antibody [GT2811] detects NS3 (Dengue virus) protein by western blot analysis.
A. 66 μg BHK-21 whole cell extract
B. 20 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 10 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
10% SDS-PAGE
NS3 (Dengue virus) antibody [GT2811] (GTX629477) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Anti-Dengue virus NS3 protein antibody [GT2811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629477

GTX629477 ICC/IF Image

NS3 (Dengue virus) antibody [GT2811] detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in paraformaldehyde.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody [GT2811] (GTX629477) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus NS3 protein antibody [GT2811] used in Western Blot (WB). GTX629477

GTX629477 WB Image

NS3 (Dengue Virus 2) antibody [GT2811] detects NS3 protein by western blot analysis.
A. 30 μg BHK21 whole cell lysate/extract
B. 30 μg whole cell lysate/extract of Dengue virus 2 infected BHK21 cells
C. 30 μg whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells
7.5% SDS-PAGE
NS3 (Dengue Virus 2) antibody [GT2811] (GTX629477) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Dengue virus NS3 protein antibody [GT2811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629477

GTX629477 ICC/IF Image

NS3 (Dengue virus) antibody [GT2811] detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in paraformaldehyde.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody [GT2811] (GTX629477) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus NS3 protein antibody [GT2811] used in Western Blot (WB). GTX629477

GTX629477 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody [GT2811] (GTX629477) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

SH Wang et al. Virulence 2023; 14 (1) : 2279355 Lambda-free light chain: A serum marker of dengue disease via NS3 protease-mediated antibody cleavage
Application : ELISA
Lai JH et al. Sci Rep 2023; 13 (1) : 20126 USP18 enhances dengue virus replication by regulating mitochondrial DNA release.
Denolly S et al. mBio 2023; 14 (5) : e0144123 Dengue virus NS1 secretion is regulated via importin-subunit β1 controlling expression of the chaperone GRp78 and targeted by the clinical drug ivermectin.
Brugier A et al. J Virol 2022; 96 (7) : e0196221 RACK1 Associates with RNA-Binding Proteins Vigilin and SERBP1 to Facilitate Dengue Virus Replication.
Application : WB
Jenn-Haung Lai et al. iScience 2021; 24 (6) : 102498 Mitochondrial CMPK2 mediates immunomodulatory and antiviral activities through IFN-dependent and IFN-independent pathways.
Application : ICC/IF
Show More +
Submit a Reference

REVIEW

Dengue virus NS3 protein antibody [GT2811] Cat. No. GTX629477

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 3 users reviews)
Anti-Dengue virus NS3 protein antibody [GT2811] used in Western Blot (WB). GTX629477
Date : Anonymous submitted on 25-Jul-2023
Score :
Application Tested : WB
Sample Species : DENV1, DENV2, DENV3, DENV4
Sample : BHK-21 celles with Dengue virus type 1/2/3/4 infection
Amount used : 5μg
Blocking : 5% non-fat milk in TBST, 25°C, 0.5-1Hr
Primary Antibody : 1:5000, 4°C, >12Hr
Figure Note : *: Non-specific band
Anti-Dengue virus NS3 protein antibody [GT2811] used in Western Blot (WB). GTX629477
Date : Anonymous submitted on 04-May-2021
Score :
Application Tested : WB
Sample : A549 cells with/without Dengue virus infection
Amount used : 10 μg
Blocking : 5% skim milk in TBST, 27°C, 1Hr
Primary Antibody : 1:5000, 4°C, 12Hr
Lane Description : Non-infected (mock) and infected (infection) A549 whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody (GTX629477) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Anti-Dengue virus NS3 protein antibody [GT2811] used in Western Blot (WB). GTX629477
Date : Anonymous submitted on 21-Nov-2013
Score :
Application Tested : WB
Sample Species : DENV1
Sample : Dengue virus 1 infected C6/36 mosquito cells
Amount used : 30μg
Blocking : 5% BSA in 1x TBS, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Lane Description : Lane A:Mock, Lane B:Dengue virus 1 infected C6/36 mosquito cells
Submit a Review
Application Reference
SH Wang et al. Virulence 2023; 14 (1):2279355 Lambda-free light chain: A serum marker of dengue disease via NS3 protease-mediated antibody cleavage
Application : ELISA
Lai JH et al. Sci Rep 2023; 13 (1):20126 USP18 enhances dengue virus replication by regulating mitochondrial DNA release.
Denolly S et al. mBio 2023; 14 (5):e0144123 Dengue virus NS1 secretion is regulated via importin-subunit β1 controlling expression of the chaperone GRp78 and targeted by the clinical drug ivermectin.
Brugier A et al. J Virol 2022; 96 (7):e0196221 RACK1 Associates with RNA-Binding Proteins Vigilin and SERBP1 to Facilitate Dengue Virus Replication.
Application : WB
Jenn-Haung Lai et al. iScience 2021; 24 (6):102498 Mitochondrial CMPK2 mediates immunomodulatory and antiviral activities through IFN-dependent and IFN-independent pathways.
Application : ICC/IF
Mazeaud C et al. Viruses 2021; 13 (10) The Biogenesis of Dengue Virus Replication Organelles Requires the ATPase Activity of Valosin-Containing Protein.
Application : WB
Sarah Goellner et al. STAR Protoc 2020; 1 (3):100173 Replication-Independent Generation and Morphological Analysis of Flavivirus Replication Organelles.
Application : WB, ICC/IF
Labeau A et al. J Virol 2020; 94 (7) A Genome-Wide CRISPR-Cas9 Screen Identifies the Dolichol-Phosphate Mannose Synthase Complex as a Host Dependency Factor for Dengue Virus Infection.
Cerikan B et al. Cell Rep 2020; 32 (1):107859 A Non-Replicative Role of the 3' Terminal Sequence of the Dengue Virus Genome in Membranous Replication Organelle Formation.
Application : ICC/IF
Ngo AM et al. Elife 2019; 8 The ER membrane protein complex is required to ensure correct topology and stable expression of flavivirus polyproteins.
Application : WB
Frakolaki E et al. Cells 2019; 8 (8) Emerging Role of l-Dopa Decarboxylase in Flaviviridae Virus Infections.
Application : WB
Cortese M et al. Cell Rep 2019; 27 (9):2579-2592.e6 Reciprocal Effects of Fibroblast Growth Factor Receptor Signaling on Dengue Virus Replication and Virion Production.
Wesley Freppel et al. Bio Protoc 2018; 8 (24):e3115 Production, Titration and Imaging of Zika Virus in Mammalian Cells.
Tohma D et al. Arch Virol 2018; (Epub) An estrogen antagonist, cyclofenil, has anti-dengue-virus activity.
Application : WB
Liu X et al. Virology 2018; 524:114-126 Homologous RNA secondary structure duplications in 3' untranslated region influence subgenomic RNA production and replication of dengue virus.
Application : ICC/IF
Lai JH et al. EMBO Rep 2018; (Epub) Infection with the dengue RNA virus activates TLR9 signaling in human dendritic cells.
Application : ICC/IF
Liu H et al. J Virol 2017; 91 (9) Endoplasmic Reticulum Protein SCAP Inhibits Dengue Virus NS2B3 Protease by Suppressing Its K27-Linked Polyubiquitylation.
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
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