Dengue virus NS4A protein antibody

Name: Dengue virus NS4A protein antibody
Brand: GeneTex
SKU: GTX132069
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Dengue virus NS4A protein antibody used in Western Blot (WB). GTX132069

Anti-Dengue virus NS4A protein antibody used in IHC (Paraffin sections) (IHC-P). GTX132069

Anti-Dengue virus NS4A protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132069

Cat. No. GTX132069

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Dengue virus 2
Package	100 μl ($399), 25 μl ($169)

See all Dengue virus NS4A protein products

View product citations for antibody GTX132069 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

16 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Full length Dengue virus NS4A recombinant protein. (Dengue virus 2 (strain 16681 PDK 53))

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886553

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Dengue Virus Non-structural protein 4A , Dengue Virus NS4A , DENV Non-structural protein 4A , DENV NS4A

Research Area

DATA IMAGES

GTX132069 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS4A protein antibody (GTX132069) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX132069 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS4A protein antibody (GTX132069) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX132069 IHC-P Image

Dengue virus NS4A protein antibody detects Dengue virus NS4A protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded BHK-21 infected with dengue virus.
Green: Dengue virus NS4A protein stained by Dengue virus NS4A protein antibody (GTX132069) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX132069 ICC/IF Image

Dengue virus NS4A protein antibody detects Dengue virus NS4A protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and infected A549 cells were fixed in 4% paraformaldehyde at RT for 1 hr.
Green: Dengue virus NS4A protein stained by Dengue virus NS4A protein antibody (GTX132069) diluted at 1:720.
Blue: nuclear staining.

REFERENCE

Application Reference

Richardson RB et al. Nat Microbiol 2018; (Epub) a crispr screen identifies ifi6 as an er-resident * effector that blocks flavivirus replication.

Application : WB

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REVIEW