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TIM17 antibody [C1C3]

Orthogonal
Anti-TIM17 antibody [C1C3] used in Western Blot (WB). GTX108280
Anti-TIM17 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108280
Anti-TIM17 antibody [C1C3] used in Western Blot (WB). GTX108280
Anti-TIM17 antibody [C1C3] used in Western Blot (WB). GTX108280

Cat. No. GTX108280

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Tim17 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TIM17. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038123

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

translocase of inner mitochondrial membrane 17A , TIM17 , TIM17A

Cellular Localization

Mitochondrion inner membrane; Multi-pass membrane protein

Background

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.

Database

Research Area

DATA IMAGES

Anti-TIM17 antibody [C1C3] used in Western Blot (WB). GTX108280

GTX108280 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
12% SDS PAGE
GTX108280 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TIM17 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108280

GTX108280 ICC/IF Image

TIM17 antibody detects TIM17 protein at mitochondria by immunofluorescent analysis.
Sample: A549 cells were fixed in ice-cold MeOH for 5 min.
Green: TIM17 protein stained by TIM17 antibody (GTX108280) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-TIM17 antibody [C1C3] used in Western Blot (WB). GTX108280

GTX108280 WB Image

Orthogonal

The data was published in the journal EMBO Mol Med in 2019. PMID: 30389680

Anti-TIM17 antibody [C1C3] used in Western Blot (WB). GTX108280

GTX108280 WB Image

Orthogonal

The data was published in the journal EMBO Mol Med in 2019. PMID: 30389680

REFERENCE

Application Reference

Richter F et al. J Cell Biol 2019; 218 (2) : 598-614 ROMO1 is a constituent of the human presequence translocase required for YME1L protease import.
Sprenger HG et al. EMBO Mol Med 2019; 11 (1) Loss of the mitochondrial i-AAA protease YME1L leads to ocular dysfunction and spinal axonopathy.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169