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JARID2 antibody

KO/KD Comparison Overexpression
Anti-JARID2 antibody used in Western Blot (WB). GTX129019
Anti-JARID2 antibody used in Western Blot (WB). GTX129019
Anti-JARID2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129019
Anti-JARID2 antibody used in Western Blot (WB). GTX129019
Anti-JARID2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129019
Anti-JARID2 antibody used in Western Blot (WB). GTX129019
Anti-JARID2 antibody used in Western Blot (WB). GTX129019

Cat. No. GTX129019

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all JARID2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Positive Control

293T , A431 , human JARID2-transfected 293T cells , fetal mouse forebrain , HeLa

Predict Reactivity

Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human JARID2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885871

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

jumonji and AT-rich interaction domain containing 2 , JMJ

Cellular Localization

Nucleus

Background

This gene is an ortholog of the mouse jumonji gene, which encodes a nuclear protein essential for mouse embryogenesis, including neural tube formation. Overexpression of mouse jumonji negatively regulates cell proliferation. The jumonji proteins contain a DNA-binding domain, called an AT-rich interaction domain (ARID), and share regions of similarity with human retinoblastoma-binding protein-2 and the human SMCX protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-JARID2 antibody used in Western Blot (WB). GTX129019

GTX129019 WB Image

KO/KD

Wild-type (WT) and JARID2 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with JARID2 antibody (GTX129019) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-JARID2 antibody used in Western Blot (WB). GTX129019

GTX129019 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with JARID2 antibody (GTX129019) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-JARID2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129019

GTX129019 IHC-P Image

JARID2 antibody detects JARID2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
JARID2 stained by JARID2 antibody (GTX129019) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-JARID2 antibody used in Western Blot (WB). GTX129019

GTX129019 WB Image

JARID2 antibody detects JARID2 protein by western blot analysis.
A. 50 μg fetal mouse forebrain lysate/extract
5 % SDS-PAGE
JARID2 antibody (GTX129019) dilution: 1:1000

Anti-JARID2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129019

GTX129019 IHC-P Image

JARID2 antibody detects JARID2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spinal cord.
JARID2 stained by JARID2 antibody (GTX129019) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-JARID2 antibody used in Western Blot (WB). GTX129019

GTX129019 WB Image

Overexpression

JARID2 antibody detects JARID2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human JARID2-transfected 293T cells
5 % SDS-PAGE
JARID2 antibody (GTX129019) dilution: 1:20000

Anti-JARID2 antibody used in Western Blot (WB). GTX129019

GTX129019 WB Image

JARID2 antibody detects JARID2 protein by Western blot analysis.
A. 30 μg 293T
B. 30 μg A431
5 % SDS-PAGE
JARID2 antibody (GTX129019) dilution: 1:1000

REFERENCE

Application Reference

Celik H et al. Cancer Cell 2018; 34 (5) : 741-756.e8 JARID2 Functions as a Tumor Suppressor in Myeloid Neoplasms by Repressing Self-Renewal in Hematopoietic Progenitor Cells.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169