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Myc tag antibody

Anti-Myc tag antibody used in Immunoprecipitation (IP). GTX115046
Anti-Myc tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115046
Anti-Myc tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115046
Anti-Myc tag antibody used in Western Blot (WB). GTX115046
Anti-Myc tag antibody used in Western Blot (WB). GTX115046
Anti-Myc tag antibody used in Western Blot (WB). GTX115046

Cat. No. GTX115046

Reference 21

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all Myc tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IP 1:10000
Not tested in other applications.

Positive Control

Transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Synthetic peptide corresponding to a region within amino acids 410 and 419 of human c-Myc

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169757

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Myc tag antibody

Research Area

DATA IMAGES

Anti-Myc tag antibody used in Immunoprecipitation (IP). GTX115046

GTX115046 IP Image

Myc antibody Immunoprecipitation of Myc tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-Myc antibody (GTX115046). (A) Input, 15ug of HeLa transfected lysates. The precipitated protein was detected by GTX115046 diluted at 1:10000. EasyBlot anti-Rabbit IgG Kit (GTX225856-01) was used in Western blot.

Anti-Myc tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115046

GTX115046 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Myc tag transfected HeLa, using Myc tag(GTX115046) antibody (green) at 1:3000 dilution. Alpha-tubulin filaments were labeled with GTX11304 (red) at 1:2500.

Anti-Myc tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115046

GTX115046 ICC/IF Image

Myc tag antibody detects Myc tag protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Myc-tag tagged protein stained by Myc tag antibody (GTX115046) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Myc tag antibody used in Western Blot (WB). GTX115046

GTX115046 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Myc tag antibody (GTX115046) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Myc tag antibody used in Western Blot (WB). GTX115046

GTX115046 WB Image

The data was published in the journal J Virol in 2016. PMID: 27489281

Anti-Myc tag antibody used in Western Blot (WB). GTX115046

GTX115046 WB Image

The data was published in the journal Oncogene in 2017. PMID: 28436950

REFERENCE

Application Reference

Healy MD et al. Cell 2023; 186 (10) : 2219-2237.e29 Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome.
Han-Yu Wang et al. Cells 2021; 10 (2) Protein Kinase A-Mediated Septin7 Phosphorylation Disrupts Septin Filaments and Ciliogenesis.
Application : WB, ICC/IF
Satoh Y et al. J Gen Virol 2021; 102 (10) M protein of subacute sclerosing panencephalitis virus, synergistically with the F protein, plays a crucial role in viral neuropathogenicity.
Application : WB
Kuge H et al. J Lipid Res 2020; 61 (12) : 1747-1763 PLRP2 selectively localizes synaptic membrane proteins via acyl-chain remodeling of phospholipids.
Liu YH et al. Biosci Rep 2020; 40 (8) Association of variant on the promoter of cluster of differentiation 74 in graves disease and graves ophthalmopathy.
Application : IP
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REVIEW

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Application Reference
Healy MD et al. Cell 2023; 186 (10):2219-2237.e29 Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome.
Han-Yu Wang et al. Cells 2021; 10 (2) Protein Kinase A-Mediated Septin7 Phosphorylation Disrupts Septin Filaments and Ciliogenesis.
Application : WB, ICC/IF
Satoh Y et al. J Gen Virol 2021; 102 (10) M protein of subacute sclerosing panencephalitis virus, synergistically with the F protein, plays a crucial role in viral neuropathogenicity.
Application : WB
Kuge H et al. J Lipid Res 2020; 61 (12):1747-1763 PLRP2 selectively localizes synaptic membrane proteins via acyl-chain remodeling of phospholipids.
Liu YH et al. Biosci Rep 2020; 40 (8) Association of variant on the promoter of cluster of differentiation 74 in graves disease and graves ophthalmopathy.
Application : IP
Lai HJ et al. J Neurol Sci 2020; 413:116810 Electrophysiological parameters that contribute to the pathogenesis of familial amyloid polyneuropathy caused by transthyretin mutations.
Application : WB
Cao C et al. Front Microbiol 2019; 10:3040 BICP0 Negatively Regulates TRAF6-Mediated NF-庥B and Interferon Activation by Promoting K48-Linked Polyubiquitination of TRAF6.
Application : WB
Te Velthuis AJW et al. Nat Microbiol 2018; 3 (11):1234-1242 Mini viral RNAs act as innate immune agonists during influenza virus infection.
Montes-Chinea NI et al. Neurol Genet 2018; 4 (6):e282 Identification of a new SYT2 variant validates an unusual distal motor neuropathy phenotype.
Application : ICC/IF
Aartjan te Velthuis et al. bioRxiv 2018; (Epub) Mini viral RNAs act as innate immune agonists during influenza virus infection.
Application : WB
von Morgen P et al. Oncogene 2017; (Epub) MRE11 stability is regulated by CK2-dependent interaction with R2TP complex.
Application : WB
Shen YR et al. PLoS Genet 2017; 13 (3):e1006631 SEPT12 phosphorylation results in loss of the septin ring/sperm annulus, defective sperm motility and poor male fertility.
Application : ICC/IF
Chang KH et al. J Formos Med Assoc 2016; Functional properties of LRRK2 mutations in Taiwanese Parkinson disease.
Application : WB
Wong MT et al. J Virol 2016; Human Choline Kinase-α Promotes Hepatitis C Virus RNA Replication through Modulating Membranous Viral Replication Complex Formation.
Application : WB
Yen CS et al. Sci Rep 2016; 6:25082 Lovastatin causes FaDu hypopharyngeal carcinoma cell death via AMPK-p63-survivin signaling cascade.
Application : IP
Ding Y et al. Dev Biol 2016; (Epub) Genome-wide analysis of dorsal and ventral transcriptomes of the Xenopus laevis gastrula.
Application : WB, IP
Kuo HY et al. Glycobiology 2016; 26 (2):155-65 Galectin-3 modulates the EGFR signalling-mediated regulation of Sox2 expression via c-Myc in lung cancer.
Lin YC et al. J Leukoc Biol 2015; Syk is involved in NLRP3 inflammasome-mediated caspase-1 activation through adaptor ASC phosphorylation and enhanced oligomerization.
Thomas S et al. Hum Mutat 2014; 35 (1):137-46 A homozygous PDE6D mutation in Joubert syndrome impairs targeting of farnesylated INPP5E protein to the primary cilium.
Application : WB
Guo YC et al. Neurology 2014; 83 (17):1554-61 Spinocerebellar ataxia 35: novel mutations in TGM6 with clinical and genetic characterization.
Application : WB
Yang JH et al. Nucleic Acids Res 2014; 42 (15):9908-24 Arginine methylation of hnRNPK negatively modulates apoptosis upon DNA damage through local regulation of phosphorylation.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169