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COL5A2 antibody, N-term

Anti-COL5A2 antibody, N-term used in Flow cytometry (FACS). GTX81507
Anti-COL5A2 antibody, N-term used in Western Blot (WB). GTX81507

Cat. No. GTX81507

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
400 μl ($399)
See all COL5A2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P Assay dependent
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

145 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 90-118 amino acids from the N-terminal region of human COL5A2.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

RRID

AB_11169696

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type V alpha 2 chain , EDSC , EDSCL2

Background

This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-COL5A2 antibody, N-term used in Flow cytometry (FACS). GTX81507

GTX81507 FACS Image

FACS analysis of NCI-H460 cells using GTX81507 COL5A2 antibody, N-term.
Top histogram : negative control
Bottom histogram : NCI-H460 cells

Anti-COL5A2 antibody, N-term used in Western Blot (WB). GTX81507

GTX81507 WB Image

WB analysis of mouse lung tissue lysate (35ug/lane) using GTX81507 COL5A2 antibody, N-term.

REFERENCE

Application Reference

Fan Yang et al. Am J Transl Res 2018; 10 (8) : 2469-2479 Collagen type V a2 (COL5A2) is decreased in steroid-induced necrosis of the femoral head.
Application : IHC-P
Reactivity : Rat
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399