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AKT antibody

Orthogonal
Anti-AKT antibody used in IHC (Paraffin sections) (IHC-P). GTX28805
Anti-AKT antibody used in Western Blot (WB). GTX28805
Anti-AKT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28805
Anti-AKT antibody used in (). GTX28805
Anti-AKT antibody used in Western Blot (WB). GTX28805
Anti-AKT antibody used in Western Blot (WB). GTX28805
Anti-AKT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28805
Anti-AKT antibody used in Western Blot (WB). GTX28805

Cat. No. GTX28805

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Rat, Chicken
Package
100 μl ($399)
See all AKT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:1000
IHC-P 1:500-1:2000
FACS Assay dependent
ELISA 1:2000-1:10000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide RPHFPQFSYSASGTA corresponding to the Cterminus (460-480) of human AKT proteins conjugated to KLH using maleimide.

Conjugation

Unconjugated

RRID

AB_367524

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Akt (Protein Kinase B, PKB) is a key regulator of many signal transduction pathways (reviewed in ref 1). Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of Akt has been seen in many types of cancer. Akt mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits Akt to the membrane, where it is activated by PDK1 phosphorylation (reviewed in 2). Once phosphorylated, Akt dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. Akt has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.

Research Area

DATA IMAGES

Anti-AKT antibody used in IHC (Paraffin sections) (IHC-P). GTX28805

GTX28805 IHC-P Image

Immunohistochemical analysis of formalin-fixed paraffin-embedded tissue, A: Normal colon tissue, B: Colon tumor tissue, using AKT(GTX28805) antibody at 1:1,000 dilution for 1 h at RT. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: AKT is nuclear. Staining: AKT as precipitated red signal with hematoxylin purple nuclear counterstain.

Anti-AKT antibody used in Western Blot (WB). GTX28805

GTX28805 WB Image

Western Blot of simultaneous detection of unphosphorylated and phosphorylated Rabbit Anti-AKT antibody (GTX28805). Lane 1: unstimulated NIH/3T3 lysates contain inactive unphosphorylated Akt1, green band. Lane 2: PDGF stimulated NIH/3T3 lysate contains both inactive (green band) and activated phosphorylated Akt1 (red band). Load: 35 μg per lane. Primary antibody: rabbit anti-Akt (pan) and mouse anti-Akt pS473 specific antibodies at 1:1000 for overnight at 4ºC. Secondary antibody: Flourescent 549 conjugated anti-rabbit IgG (green) and flourescent 649 conjugated anti-mouse IgG (red) secondary antibodies at 1:10,000 for 45 min at RT.

Anti-AKT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28805

GTX28805 ICC/IF Image

ICC/IF of the Akt antibody is used at 1: 80 for 1hr at RT on cultured neonatal rat cardiomyocytes (Green). The red is Texas-red phalloidin that labels actin filaments.

Anti-AKT antibody used in (). GTX28805

GTX28805 Image

Anti-AKT antibody used in Western Blot (WB). GTX28805

GTX28805 WB Image

Western blot using Akt antibody at 1:500 for overnight at 4ºC on 20 ug NIH/3T3 whole cell lysate.

Anti-AKT antibody used in Western Blot (WB). GTX28805

GTX28805 WB Image

WB analysis of NIH-3T3 whole cell lysate using GTX28805 AKT antibody.
Loading : 20 μg
Dilution : 1:500

Anti-AKT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28805

GTX28805 ICC/IF Image

ICC/IF analysis of PFA-fixed rat neonatal cardiomyocytes using GTX28805 AKT antibody.
Green : Primary antibody
Red : Actin
Dilution : 1:80

Anti-AKT antibody used in Western Blot (WB). GTX28805

GTX28805 WB Image

Orthogonal

WB analysis of PDGF treated and untreated NIH-3T3 whole cell lysate using GTX28805 AKT antibody.
Green : Primary antibody
Red : Akt (phospho Ser473)
Lane 1 : Untreated whole cell lysate
Lane 2 : PDGF-treated whole cell lysate
Loading : 35 μg
Dilution : 1:1000

REFERENCE

Application Reference

Zhou W et al. Front Pharmacol 2024; 15 : 1290128 Neuroprotective effects of a novel peptide through the Rho-integrin-Tie2 and PI3K/Akt pathways in experimental autoimmune encephalomyelitis model.
Li M et al. Dis Markers 2022; 2022 : 1941077 DDTC Suppresses Ovarian Cancer Development via the PI3K/AKT/mTOR Signaling Pathway.
Application : WB
Reactivity : Human
Meng Li et al. Dis Markers 2021; 2021 : 4304507 The Mechanism of Xiaoyao San in the Treatment of Ovarian Cancer by Network Pharmacology and the Effect of Stigmasterol on the PI3K/Akt Pathway.
Application : WB
Reactivity : Human
Zhu M et al. Ecotoxicol Environ Saf 2021; 214 : 112091 MAPK, AKT/FoxO3a and mTOR pathways are involved in cadmium regulating the cell cycle, proliferation and apoptosis of chicken follicular granulosa cells.
Application : WB
Zhao J et al. J Steroid Biochem Mol Biol 2018; (Epub) Orchiectomy and * differentially regulate synaptic plasticity and spatial memory in a manner that is mediated by SRC-1 in the hippocampus of male mice.
Application : WB
Reactivity : Mouse
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399