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GAD67 antibody

Overexpression
Anti-GAD67 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113190
Anti-GAD67 antibody used in Western Blot (WB). GTX113190
Anti-GAD67 antibody used in IHC (Paraffin sections) (IHC-P). GTX113190
Anti-GAD67 antibody used in Western Blot (WB). GTX113190
Anti-GAD67 antibody used in IHC (Paraffin sections) (IHC-P). GTX113190
Anti-GAD67 antibody used in Western Blot (WB). GTX113190
Anti-GAD67 antibody used in IHC (Paraffin sections) (IHC-P). GTX113190

Cat. No. GTX113190

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GAD67 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Bovine, Cat, Dog, Chicken, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GAD67. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618194

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate decarboxylase 1 , CPSQ1 , GAD , SCP

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GAD67 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113190

GTX113190 ICC/IF Image

GAD67 antibody detects GAD67 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAD67 protein stained by GAD67 antibody (GTX113190) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-GAD67 antibody used in Western Blot (WB). GTX113190

GTX113190 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX113190) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD67 antibody used in IHC (Paraffin sections) (IHC-P). GTX113190

GTX113190 IHC-P Image

GAD67 antibody detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GAD67 stained by GAD67 antibody (GTX113190) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAD67 antibody used in Western Blot (WB). GTX113190

GTX113190 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX113190) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD67 antibody used in IHC (Paraffin sections) (IHC-P). GTX113190

GTX113190 IHC-P Image

GAD67 antibody detects GAD67 protein expression by immunohistochemical analysis.
Sample: Paraffin-Embedded adult mouse retina.
Green: GAD67 protein stained by GAD67 antibody (GTX113190) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAD67 antibody used in Western Blot (WB). GTX113190

GTX113190 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX113190) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-GAD67 antibody used in IHC (Paraffin sections) (IHC-P). GTX113190

GTX113190 IHC-P Image

GAD67 antibody detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GAD67 stained by GAD67 antibody (GTX113190) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Mana Naito et al. Biomedicines 2022; 10 (2) Immunohistochemical Analysis of Neurotransmitters in Neurosecretory Protein GL-Producing Neurons of the Mouse Hypothalamus.
Application : IHC-Fr
Reactivity : Mouse
Kwon O et al. Cells 2021; 10 (10) TWIK-1 BAC-GFP Transgenic Mice, an Animal Model for TWIK-1 Expression.
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169