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GNAT2 antibody

Comparison
Anti-GNAT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134342
Anti-GNAT2 antibody used in Western Blot (WB). GTX134342
Anti-GNAT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX134342

Cat. No. GTX134342

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GNAT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

mouse eye

Predict Reactivity

Human, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human GNAT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887254

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein subunit alpha transducin 2 , ACHM4 , GNATC

Background

Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in cones. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GNAT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134342

GTX134342 ICC/IF Image

GNAT2 antibody detects GNAT2 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GNAT2 stained by GNAT2 antibody (GTX134342) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GNAT2 antibody used in Western Blot (WB). GTX134342

GTX134342 WB Image

Comparison

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membranes were blotted with GNAT2 antibody (GTX134342) diluted at 1:1000 and competitor's antibody (sc-390) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-GNAT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX134342

GTX134342 IHC-P Image

GNAT2 antibody detects GNAT2 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: GNAT2 stained by GNAT2 antibody (GTX134342) diluted at 1:200.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Son Y et al. Cell Death Dis 2022; 13 (9) : 833 Mdm1 ablation results in retinal degeneration by specific intraflagellar transport defects of photoreceptor cells.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169