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HMGA2 antibody [GT763]

Anti-HMGA2 antibody [GT763] used in IHC (Paraffin sections) (IHC-P). GTX629478
Anti-HMGA2 antibody [GT763] used in Immunoprecipitation (IP). GTX629478
Anti-HMGA2 antibody [GT763] used in IHC (Paraffin sections) (IHC-P). GTX629478
Anti-HMGA2 antibody [GT763] used in Western Blot (WB). GTX629478
Anti-HMGA2 antibody [GT763] used in Western Blot (WB). GTX629478
Anti-HMGA2 antibody [GT763] used in Western Blot (WB). GTX629478
Anti-HMGA2 antibody [GT763] used in IHC (Paraffin sections) (IHC-P). GTX629478
Anti-HMGA2 antibody [GT763] used in IHC (Paraffin sections) (IHC-P). GTX629478
Anti-HMGA2 antibody [GT763] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629478
Anti-HMGA2 antibody [GT763] used in IHC (Paraffin sections) (IHC-P). GTX629478
Anti-HMGA2 antibody [GT763] used in Western Blot (WB). GTX629478

Cat. No. GTX629478

Host

Mouse

Clonality

Monoclonal

Clone Name

GT763

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

HepG2 , Rat-2 , NIH-3T3

Predict Reactivity

Zebrafish, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMGA2. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888116

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. Due to intellectual property restrictions, this product is not available for purchase in AT, BE, CH, DE, DK, ES, FR, GB, GR, IE, IT, LI, LU, MC, NL, PT, SE.

TARGET

Synonyms

high mobility group AT-hook 2 , BABL , HMGI-C , HMGIC , LIPO , STQTL9

Cellular Localization

Nucleus

Background

This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HMGA2 antibody [GT763] used in IHC (Paraffin sections) (IHC-P). GTX629478

GTX629478 IHC-P Image

HMGA2 antibody [GT763] detects HMGA2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
HMGA2 stained by HMGA2 antibody [GT763] (GTX629478) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGA2 antibody [GT763] used in Immunoprecipitation (IP). GTX629478

GTX629478 IP Image

Immunoprecipitation of HMGA2 protein from HepG2 nuclear extracts using 5 μg of HMGA2 antibody [GT763] (GTX629478).
Western blot analysis was performed using HMGA2 antibody [GT763] (GTX629478) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-HMGA2 antibody [GT763] used in IHC (Paraffin sections) (IHC-P). GTX629478

GTX629478 IHC-P Image

HMGA2 antibody [GT763] detects HMGA2 protein on C2C12 by immunohistochemical analysis.
Sample: Paraffin-embedded C2C12.
HMGA2 antibody [GT763] (GTX629478) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGA2 antibody [GT763] used in Western Blot (WB). GTX629478

GTX629478 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody [GT763] (GTX629478) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HMGA2 antibody [GT763] used in Western Blot (WB). GTX629478

GTX629478 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody [GT763] (GTX629478) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HMGA2 antibody [GT763] used in Western Blot (WB). GTX629478

GTX629478 WB Image

HMGA2 antibody [GT763] detects HMGA2 protein by western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract
15% SDS-PAGE
HMGA2 antibody [GT763] (GTX629478) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HMGA2 antibody [GT763] used in IHC (Paraffin sections) (IHC-P). GTX629478

GTX629478 IHC-P Image

HMGA2 antibody [GT763] detects HMGA2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
HMGA2 stained by HMGA2 antibody [GT763] (GTX629478) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HMGA2 antibody [GT763] used in IHC (Paraffin sections) (IHC-P). GTX629478

GTX629478 IHC-P Image

HMGA2 antibody [GT763] detects HMGA2 protein at on RT2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded RT2 xenograft.
HMGA2 antibody [GT763] (GTX629478) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGA2 antibody [GT763] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629478

GTX629478 ICC/IF Image

HMGA2 antibody [GT763] detects HMGA2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGA2 protein stained by HMGA2 antibody [GT763] (GTX629478) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.

Anti-HMGA2 antibody [GT763] used in IHC (Paraffin sections) (IHC-P). GTX629478

GTX629478 IHC-P Image

HMGA2 antibody [GT763] detects HMGA2 protein at on SkHep1 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SkHep1 xenograft.
HMGA2 antibody [GT763] (GTX629478) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGA2 antibody [GT763] used in Western Blot (WB). GTX629478

GTX629478 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody [GT763] (GTX629478) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations