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1 .GTX115549 Histone H3.3B antibody

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Histone H3.3B antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, WB
  • Species reactivityHuman, Mouse, Rat
  • Publication  ( 2 )
Cat No: GTX115549
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
  • Anti-Histone H3.3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115549
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
  • Anti-Histone H3.3B antibody used in Western Blot (WB). GTX115549
  • Anti-Histone H3.3B antibody used in Western Blot (WB). GTX115549
  • Anti-Histone H3.3B antibody used in Western Blot (WB). GTX115549
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
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  • Application Information: Histone H3.3B antibody

Catalog Number GTX115549
☆☆☆☆☆
( 0 )
Publication ( 2 )
Product Name Histone H3.3B antibody
See all Histone H3 products
ApplicationsICC/IF, IHC-P, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:20000* Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size15 kDa (note) IsoForm (2)
Positive Controls293T , A431 , HeLa , HepG2 , Mouse brain , rat brain
Form SuppliedLiquid
Concentration0.16 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: Histone H3.3B antibody

Full NameH3 histone, family 3B (H3.3B)
BackgroundHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is poyadenylated, unlike most histone genes. The protein encoded is a member of the histone H3 family. [provided by RefSeq]
SynonymsH3.3B antibody, H3F3A antibody, H3F3B antibody, histone H3.3 antibody, H3 histone, family 3A antibody, H3 histone, family 3B (H3.3B) antibody
Cellular LocalizationNucleus
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenFull length human Histone H3.3B Recombinant protein.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Xenopus laevis, Chicken, Chimpanzee, Bovine
Predict Reactivity NoteXenopus laevis (100%), Chicken (100%), Chimpanzee (100%), Bovine (100%)
ConjugationUnconjugated

  • Storage Conditions: Histone H3.3B antibody

Storage Buffer1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Histone H3.3B antibody validated data
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
    • GTX115549 IHC-P Image
      Histone H3.3B antibody detects Histone H3.3B protein at nucleus in mouse muscle by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse muscle.
      Histone H3.3B antibody (GTX115549) diluted at 1:500.
      Top
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
    • GTX115549 IHC-P Image
      Histone H3.3B antibody detects Histone H3.3B protein at nucleus in rat kidney by immunohistochemical analysis.
      Sample: Paraffin-embedded rat kidney.
      Histone H3.3B antibody (GTX115549) diluted at 1:500.
      Top
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
    • GTX115549 IHC-P Image
      Histone H3.3B antibody detects Histone H3.3B protein at nucleus in mouse kidney by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse kidney.
      Histone H3.3B antibody (GTX115549) diluted at 1:500.
      Top
  • Anti-Histone H3.3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115549
    • GTX115549 ICC/IF Image
      Histone H3.3B antibody detects Histone H3.3B protein at nucleus by immunofluorescent analysis.
      Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: Histone H3.3B protein stained by Histone H3.3B antibody (GTX115549) diluted at 1:500.
      Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
      Top
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
    • GTX115549 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using Histone H3.3B(GTX115549) antibody at 1:500 dilution.
      Top
  • Anti-Histone H3.3B antibody used in Western Blot (WB). GTX115549
    • GTX115549 WB Image
      Sample (50 µg of whole cell lysate)
      A: mouse brain
      15% SDS PAGE
      GTX115549 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-Histone H3.3B antibody used in Western Blot (WB). GTX115549
    • GTX115549 WB Image
      Histone H3.3B antibody detects H3F3B protein by western blot analysis.
      A. 50 µg rat brain lysate/extract
      15% SDS-PAGE
      Histone H3.3B antibody (GTX115549) dilution: 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-Histone H3.3B antibody used in Western Blot (WB). GTX115549
    • GTX115549 WB Image
      Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3.3B antibody (GTX115549) diluted at 1:10000.
      Top
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
    • GTX115549 IHC-P Image
      Histone H3.3B antibody detects Histone H3.3B protein at nucleus in rat brain by immunohistochemical analysis.
      Sample: Paraffin-embedded rat brain.
      Histone H3.3B antibody (GTX115549) diluted at 1:500.
      Top
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
    • GTX115549 IHC-P Image
      Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse colon by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse colon.
      Histone H3.3B antibody (GTX115549) dilution: 1:500.
      Top
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
    • GTX115549 IHC-P Image
      Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse prostate by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse prostate.
      Histone H3.3B antibody (GTX115549) dilution: 1:500.
      Top
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
    • GTX115549 IHC-P Image
      Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse lung by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse lung.
      Histone H3.3B antibody (GTX115549) diluted at 1:500.
      Top
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
    • GTX115549 IHC-P Image
      Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse spinal cord by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse spinal cord.
      Histone H3.3B antibody (GTX115549) diluted at 1:500.
      Top
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
    • GTX115549 IHC-P Image
      Histone H3.3B antibody detects Histone H3.3B protein at nucleus in mouse muscle by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse muscle.
      Histone H3.3B antibody (GTX115549) diluted at 1:500.
      Top
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
    • GTX115549 IHC-P Image
      Histone H3.3B antibody detects Histone H3.3B protein at nucleus in rat kidney by immunohistochemical analysis.
      Sample: Paraffin-embedded rat kidney.
      Histone H3.3B antibody (GTX115549) diluted at 1:500.
      Top
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
    • GTX115549 IHC-P Image
      Histone H3.3B antibody detects Histone H3.3B protein at nucleus in mouse kidney by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse kidney.
      Histone H3.3B antibody (GTX115549) diluted at 1:500.
      Top
  • Anti-Histone H3.3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115549
    • GTX115549 ICC/IF Image
      Histone H3.3B antibody detects Histone H3.3B protein at nucleus by immunofluorescent analysis.
      Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: Histone H3.3B protein stained by Histone H3.3B antibody (GTX115549) diluted at 1:500.
      Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
      Top
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
    • GTX115549 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using Histone H3.3B(GTX115549) antibody at 1:500 dilution.
      Top
  • Anti-Histone H3.3B antibody used in Western Blot (WB). GTX115549
    • GTX115549 WB Image
      Sample (50 µg of whole cell lysate)
      A: mouse brain
      15% SDS PAGE
      GTX115549 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-Histone H3.3B antibody used in Western Blot (WB). GTX115549
    • GTX115549 WB Image
      Histone H3.3B antibody detects H3F3B protein by western blot analysis.
      A. 50 µg rat brain lysate/extract
      15% SDS-PAGE
      Histone H3.3B antibody (GTX115549) dilution: 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-Histone H3.3B antibody used in Western Blot (WB). GTX115549
    • GTX115549 WB Image
      Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3.3B antibody (GTX115549) diluted at 1:10000.
      Top
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
    • GTX115549 IHC-P Image
      Histone H3.3B antibody detects Histone H3.3B protein at nucleus in rat brain by immunohistochemical analysis.
      Sample: Paraffin-embedded rat brain.
      Histone H3.3B antibody (GTX115549) diluted at 1:500.
      Top
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
    • GTX115549 IHC-P Image
      Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse colon by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse colon.
      Histone H3.3B antibody (GTX115549) dilution: 1:500.
      Top
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
    • GTX115549 IHC-P Image
      Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse prostate by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse prostate.
      Histone H3.3B antibody (GTX115549) dilution: 1:500.
      Top
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
    • GTX115549 IHC-P Image
      Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse lung by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse lung.
      Histone H3.3B antibody (GTX115549) diluted at 1:500.
      Top
  • Anti-Histone H3.3B antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115549
    • GTX115549 IHC-P Image
      Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse spinal cord by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse spinal cord.
      Histone H3.3B antibody (GTX115549) diluted at 1:500.
      Top
Back to Top
  • Reference for Histone H3.3B antibody
Application References Application Reactivity
Lee LC et al. Role of high mobility group box 1 (HMGB1) in SCA17 pathogenesis. PLoS One 9 (12):e115809 (2014). WB Human
Chang KH et al. Aqueous Extract of Paeonia lactiflora and Paeoniflorin as Aggregation Reducers Targeting Chaperones in Cell Models of Spinocerebellar Ataxia 3. Evid Based Complement Alternat Med 2013:471659 (2013). WB Human
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensH3F3B3021P84243, B2R4P9601058BC001124AAH0112415.33
Homo sapiensH3F3B3021P84243, B2R4P9601058NM_005324NP_00531515.33

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusH3f3b15081P84244, A2A852NM_008211NP_03223715.33
Rattus norvegicusH3f3b117056B0BMY8, P84245NM_053985NP_44643715.33
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-ProClin.pdf
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