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Catalog Number
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GTX115549
(
3
)
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Product Name
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Histone H3.3B antibody
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| Applications | ICC/IF, IHC-P, WB |
| Application Note |
| Suggested dilution | Reference |
| Immunocytochemistry/ Immunofluorescence (ICC/IF) | 1:100-1:1000* |
|
| IHC (Formalin-fixed paraffin-embedded sections) | 1:100-1:1000* |
|
| Western Blot (WB) | 1:500-1:20000* |
Y |
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
|
| Predicted Target Size | 15 kDa (note)
 (2)
|
| Positive Controls | 293T , A431 , HeLa , HepG2 , Mouse brain , rat brain |
| Form Supplied | Liquid |
| Concentration | 0.16
mg/ml
(Please refer to the vial label for the specific concentration) |
| Purification | Purified by antigen-affinity chromatography. |
| Full Name | H3 histone, family 3B (H3.3B) |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is poyadenylated, unlike most histone genes. The protein encoded is a member of the histone H3 family. [provided by RefSeq] |
| Synonyms | H3.3B antibody, H3F3A antibody, H3F3B antibody, histone H3.3 antibody, H3 histone, family 3A antibody, H3 histone, family 3B (H3.3B) antibody |
| Cellular Localization | Nucleus |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Full length human Histone H3.3B Recombinant protein. |
| Antigen Species | Human |
| Species Reactivity | Human, Mouse, Rat |
Predicted Cross Reactivity species  Predicted cross-reactivity:
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
| Xenopus laevis, Chicken, Chimpanzee, Bovine |
| Predict Reactivity Note | Xenopus laevis (100%), Chicken (100%), Chimpanzee (100%), Bovine (100%) |
| Conjugation | Unconjugated |