Histone H4K8ac (acetyl Lys8) antibody

Name: Histone H4K8ac (acetyl Lys8) antibody
Brand: GeneTex
SKU: GTX128957
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Histone H4K8ac (acetyl Lys8) antibody used in Dot blot (Dot). GTX128957

Anti-Histone H4K8ac (acetyl Lys8) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128957

Anti-Histone H4K8ac (acetyl Lys8) antibody used in IHC (Paraffin sections) (IHC-P). GTX128957

Anti-Histone H4K8ac (acetyl Lys8) antibody used in ChIP assay (ChIP assay). GTX128957

Anti-Histone H4K8ac (acetyl Lys8) antibody used in Immunoprecipitation (IP). GTX128957

Anti-Histone H4K8ac (acetyl Lys8) antibody used in Western Blot (WB). GTX128957

Cat. No. GTX128957

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP, Dot, ChIP assay, IHC (Free Floating)
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Histone H4 products

View product citations for antibody GTX128957 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:5000
Dot	Assay dependent
ChIP assay	Assay dependent
IHC (Free Floating)	Assay dependent

Not tested in other applications.

Positive Control

HeLa (0.012% DMSO treatment for 18 hr) , HeLa (0.4 μM Trichostatin A treatment for 18 hr)

Predict Reactivity

Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding acetyl Lys8 of human Histone H4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885846

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

GTX128957 Dot Image

Dotblot analysis of anti-Histone H4 (acetyl Lys9) antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H4 (acetyl Lys9) antibody (GTX128957) 1:5000 dilution.
A: Peptide samples of H4K8 un
B: Peptide samples of H4K8ac
C: Peptide samples of H4K16un
D: Peptide samples of H4K16ac

GTX128957 ICC/IF Image

Histone H4 (acetyl Lys8) antibody detects Histone H4 (acetyl Lys8) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H4 (acetyl Lys8) protein stained by Histone H4 (acetyl Lys8) antibody (GTX128957) diluted at 1:500.
Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by GTX628802 diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX128957 IHC-P Image

Histone H4K8ac (acetyl Lys8) antibody detects Histone H4K8ac (acetyl Lys8) protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Histone H4K8ac (acetyl Lys8) antibody (GTX128957) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX128957 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 μM for 18 h) and 5 μg of either control rabbit IgG or anti-Histone H4K8ac (acetyl Lys8) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or TEXT12.

GTX128957 IP Image

Histone H4 (acetyl K9) antibody immunoprecipitates Histone H4 (acetyl K9) protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Histone H4 (acetyl K9) protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of Histone H4 (acetyl K9) protein by 2.5 μg of Histone H4 (acetyl K9) antibody (GTX128957). 12% SDS-PAGE The immunoprecipitated Histone H4 (acetyl K9) protein was detected by Histone H4 (acetyl K9) antibody (GTX128957) diluted at 1 : 5000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX128957 IHC-P Image

Histone H4K8ac (acetyl Lys8) antibody detects Histone H4K8ac (acetyl Lys8) protein at nucleus on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Histone H4K8ac (acetyl Lys8) antibody (GTX128957) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX128957 IHC-P Image

GTX128957 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4K8ac (acetyl Lys8) antibody (GTX128957) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Uboveja A et al. bioRxiv 2024; αKG-mediated carnitine synthesis promotes homologous recombination via histone acetylation.

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