|Applications||FACS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB|
Not tested in other applications.
| Immunohistochemistry||Assay-dependent dilution
|IHC (Frozen sections)||1:100-1:1000*
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||17 kDa (note)
|Positive Controls||THP-1 , HL-60 , mouse liver, rat liver|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||allograft inflammatory factor 1|
|Product Description||Rabbit Polyclonal antibody to Iba1 (allograft inflammatory factor 1)|
|Background||This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]|
|Synonyms||AIF-1 antibody, IBA1 antibody, IRT-1 antibody, IRT1 antibody, AIF1 antibody, ionized calcium-binding adapter molecule 1 antibody, allograft inflammatory factor 1 antibody, interferon gamma responsive transcript antibody, protein G1 antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human Iba1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Pig, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Pig (93%), Rhesus Monkey (99%), Bovine (88%)|