|Applications||ICC/IF, IHC, IHC-P, WB|
Not tested in other applications.
| Immunohistochemistry||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||87 kDa (note)
|Positive Controls||HeLa , U87-MG , SK-N-SH , SK-N-AS , MCF-7 , MDA-MB-231 , mouse lung , 293T transfected with LOXL2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||lysyl oxidase-like 2|
|Product Description||Rabbit Polyclonal antibody to LOXL2 (lysyl oxidase-like 2)|
|Background||This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq]|
|Synonyms||LOR2 antibody, WS9-14 antibody, LOXL2 antibody, lysyl oxidase-related protein 2 antibody, lysyl oxidase homolog 2 antibody, lysyl oxidase-like protein 2 antibody, lysyl oxidase-related protein WS9-14 antibody, lysyl oxidase related 2 antibody, lysyl oxidase-like 2 antibody|
|Cellular Localization||Secreted , extracellular space |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human LOXL2. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Xenopus laevis, Chicken, Bovine|
|Predict Reactivity Note||Rat (91%), Xenopus laevis (83%), Chicken (87%), Bovine (96%)|