PHGDH antibody [N1N2], N-term

Name: PHGDH antibody [N1N2], N-term
Brand: GeneTex
SKU: GTX101948
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-PHGDH antibody [N1N2], N-term used in Western Blot (WB). GTX101948

Anti-PHGDH antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX101948

Anti-PHGDH antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101948

Anti-PHGDH antibody [N1N2], N-term used in IHC (Frozen sections) (IHC-Fr). GTX101948

Anti-PHGDH antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101948

Cat. No. GTX101948

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all PHGDH products

View product citations for antibody GTX101948 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431 , HeLaS3 , HeLa

Predict Reactivity

Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PHGDH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951217

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoglycerate dehydrogenase , 3-PGDH , 3PGDH , HEL-S-113 , NLS , NLS1 , PDG , PGAD , PGD , PGDH , PHGDHD , SERA

Background

3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM]

Database

Gene ID: 26227 PHGDH
UniProt: O43175 PHGDH

Research Area

DATA IMAGES

GTX101948 WB Image

Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N1N2], N-term (GTX101948) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101948 IP Image

Immunoprecipitation of PHGDH protein from 293T whole cell extracts using 5 μg of PHGDH antibody [N1N2], N-term (GTX101948).
Western blot analysis was performed using PHGDH antibody [N1N2], N-term (GTX101948).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101948 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using PHGDH(GTX101948) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101948 IHC-Fr Image

PHGDH antibody [N1N2], N-term detects PHGDH protein by immunohistochemical analysis.
Sample: Frozen-sectioned rat E13.5 brain.
Green: PHGDH stained by PHGDH antibody [N1N2], N-term (GTX101948) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101948 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX101948 diluted at 1:1000

GTX101948 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:A431(GTX27909)
C:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX101948 diluted at 1:1000

GTX101948 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PHGDH(GTX101948) antibody at 1:500 dilution.

GTX101948 IHC-P Image

PHGDH antibody [N1N2], N-term detects PHGDH protein at cytoplasm on mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
PHGDH antibody [N1N2], N-term (GTX101948) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Denisa Hathazi et al. Brain 2021; INPP5K and SIL1 associated pathologies with overlapping clinical phenotypes converge through dysregulation of PHGDH.

Reactivity : Human

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REVIEW