ATG5 antibody [N1C3]

Name: ATG5 antibody [N1C3]
Brand: GeneTex
SKU: GTX102361
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-ATG5 antibody [N1C3] used in Western Blot (WB). GTX102361

Anti-ATG5 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102361

Cat. No. GTX102361

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse, Mosquito
Package	100 μl ($399), 25 μl ($169)

See all ATG5 products

View product citations for antibody GTX102361 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

Mouse brain

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATG5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240491

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy related 5 , APG5 , APG5-LIKE , APG5L , ASP , SCAR25 , hAPG5

Cellular Localization

Cytoplasm

Database

Gene ID: 9474 ATG5
UniProt: Q9H1Y0 ATG5

Research Area

DATA IMAGES

GTX102361 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX102361 diluted at 1:500

GTX102361 IHC-P Image

ATG5 antibody [N1C3] detects ATG5 protein at cytoplasm in human oral carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
ATG5 antibody [N1C3] (GTX102361) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Chiang-Wen Lee et al. Molecules 2022; 27 (2) Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.

Application : WB

Reactivity : Human

Lee CW et al. Molecules 2022; 27 (2) : 373 Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.

Application : WB

Reactivity : Human

Weng SC et al. Insect Mol Biol 2020; 29 (4) : 404-416 The unfolded protein response modulates the autophagy-mediated egg production in the mosquito Aedes aegypti.

Application : WB

Reactivity : Mosquito

Wei RJ et al. Toxicol Appl Pharmacol 2016; (Epub) A microtubule inhibitor, ABT-751, induces autophagy and delays apoptosis in Huh-7 cells.

Application : WB

Reactivity : Human

Chen SY et al. Autophagy 2011; 7 (2) : 217-28 zVAD-induced autophagic cell death requires c-Src-dependent ERK and JNK activation and reactive oxygen species generation.

Application : WB

Reactivity : Mouse

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