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CCR8 antibody

Orthogonal
Anti-CCR8 antibody used in IHC (Paraffin sections) (IHC-P). GTX100343
Anti-CCR8 antibody used in IHC (Paraffin sections) (IHC-P). GTX100343
Anti-CCR8 antibody used in Western Blot (WB). GTX100343

Cat. No. GTX100343

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CCR8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

K562

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human CCR8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240569

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-C motif chemokine receptor 8 , CC-CKR-8 , CCR-8 , CDw198 , CKRL1 , CMKBR8 , CMKBRL2 , CY6 , GPRCY6 , TER1

Cellular Localization

Cell membrane

Background

This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are important for the migration of various cell types into the inflammatory sites. This receptor protein preferentially expresses in the thymus. I-309, thymus activation-regulated cytokine (TARC) and macrophage inflammatory protein-1 beta (MIP-1 beta) have been identified as ligands of this receptor. Studies of this receptor and its ligands suggested its role in regulation of monocyte chemotaxis and thymic cell apoptosis. More specifically, this receptor may contribute to the proper positioning of activated T cells within the antigenic challenge sites and specialized areas of lymphoid tissues. This gene is located at the chemokine receptor gene cluster region. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CCR8 antibody used in IHC (Paraffin sections) (IHC-P). GTX100343

GTX100343 IHC-P Image

CCR8 antibody detects CCR8 protein at membrane on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
CCR8 antibody (GTX100343) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CCR8 antibody used in IHC (Paraffin sections) (IHC-P). GTX100343

GTX100343 IHC-P Image

CCR8 antibody detects CCR8 protein at cytosol and membrane on human hepatoma by immunohistochemical analysis.
Sample: CCR8 antibody (GTX100343) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CCR8 antibody used in Western Blot (WB). GTX100343

GTX100343 WB Image

Orthogonal

Various unboiled whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR8 antibody (GTX100343) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

S Li et al. J Cancer 2024; 15 (3) : 632-644 Exploring Prognostic Immune Microenvironment-Related Genes in Head and Neck Squamous Cell Carcinoma from the TCGA Database
Application : IHC-P
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169