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CDC2 antibody [N1], N-term

Orthogonal IP/MS
Anti-CDC2 antibody [N1], N-term used in Western Blot (WB). GTX108120
Anti-CDC2 antibody [N1], N-term used in Western Blot (WB). GTX108120
Anti-CDC2 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108120
Anti-CDC2 antibody [N1], N-term used in Western Blot (WB). GTX108120
Anti-CDC2 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108120
Anti-CDC2 antibody [N1], N-term used in Western Blot (WB). GTX108120
Anti-CDC2 antibody [N1], N-term used in Western Blot (WB). GTX108120

Cat. No. GTX108120

Reference 22
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CDC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , NIH-3T3 , JC , BCL-1 , PC-12

Product Note

IP/MS validation was supported by references (PMID:30377391)

Predict Reactivity

Drosophila(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human CDC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949938

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase 1 , CDC2 , CDC28A , P34CDC2

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CDC2 antibody [N1], N-term used in Western Blot (WB). GTX108120

GTX108120 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC2 antibody [N1], N-term (GTX108120) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDC2 antibody [N1], N-term used in Western Blot (WB). GTX108120

GTX108120 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
10% SDS PAGE
GTX108120 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDC2 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108120

GTX108120 ICC/IF Image

CDC2 antibody [N1], N-term detects CDC2 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF7 cells were fixed in ice-cold MeOH for 5 min.
Green: CDC2 protein stained by CDC2 antibody [N1], N-term (GTX108120) diluted at 1:750.
Blue: Hoechst 33342 staining.

Anti-CDC2 antibody [N1], N-term used in Western Blot (WB). GTX108120

GTX108120 WB Image

Sample (30 μg of whole cell lysate)
A: PC-12
10% SDS PAGE
GTX108120 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDC2 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108120

GTX108120 IHC-P Image

CDC2 antibody [N1], N-term detects CDC2 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
CDC2 antibody [N1], N-term (GTX108120) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CDC2 antibody [N1], N-term used in Western Blot (WB). GTX108120

GTX108120 WB Image

Sample (30 μg of whole cell lysate)
A: A549
B: H1299
C: HCT116
10% SDS PAGE
GTX108120 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDC2 antibody [N1], N-term used in Western Blot (WB). GTX108120

GTX108120 WB Image

Orthogonal

The data was published in the 2022 in FEBS Open Bio. PMID: 34856073

REFERENCE

Application Reference

Y He et al. Int J Biol Sci 2024; 20 (7) : 2403-2421 Ciliogenesis-associated Kinase 1 Promotes Breast Cancer Cell Proliferation and Chemoresistance via Phosphorylating ERK1
YY Hsieh et al. Mol Oncol 2023; Repositioning VU-0365114 as a novel microtubule-destabilizing agent for treating cancer and overcoming drug resistance
Yoshida K et al. Sci Rep 2023; 13 (1) : 15574 Public RNA-seq data-based identification and functional analyses reveal that MXRA5 retains proliferative and migratory abilities of dental pulp stem cells.
CP Chen et al. Biomed Pharmacother 2023; 166 : 115389
Application : WB
Yongfeng Zhou et al. FEBS Open Bio 2022; 12 (2) : 379-393 Integrative investigation of the TF-miRNA coregulatory network involved in the inhibition of breast cancer cell proliferation by resveratrol.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

CDC2 antibody [N1], N-term Cat. No. GTX108120

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-CDC2 antibody [N1], N-term used in Western Blot (WB). GTX108120
Date : Anonymous submitted on 19-Dec-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : HSC3 and SAS cells
Amount used : 30μg
Blocking : 5% non-fat milk, BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 15Hr
Submit a Review
Application Reference
Y He et al. Int J Biol Sci 2024; 20 (7):2403-2421 Ciliogenesis-associated Kinase 1 Promotes Breast Cancer Cell Proliferation and Chemoresistance via Phosphorylating ERK1
YY Hsieh et al. Mol Oncol 2023; Repositioning VU-0365114 as a novel microtubule-destabilizing agent for treating cancer and overcoming drug resistance
Yoshida K et al. Sci Rep 2023; 13 (1):15574 Public RNA-seq data-based identification and functional analyses reveal that MXRA5 retains proliferative and migratory abilities of dental pulp stem cells.
CP Chen et al. Biomed Pharmacother 2023; 166:115389
Application : WB
Xiaobo Wang et al. J Radiat Res 2022; 63 (3):331-341 Overcoming radio-resistance in esophageal squamous cell carcinoma via hypermethylation of PIK3C3 promoter region mediated by KDM5B loss.
Application : WB
Reactivity : Human
Yongfeng Zhou et al. FEBS Open Bio 2022; 12 (2):379-393 Integrative investigation of the TF-miRNA coregulatory network involved in the inhibition of breast cancer cell proliferation by resveratrol.
Application : WB
Reactivity : Human
Yanqi Xie et al. Mol Cancer Ther 2021; 20 (10):1893-1903 Potent Synergistic Effect on C-Myc-Driven Colorectal Cancers Using a Novel Indole-Substituted Quinoline with a Plk1 Inhibitor.
Application : WB
Reactivity : Human
Kuo TT et al. ACS Omega 2020; 5 (49):31792-31800 Melissa officinalis Extract Induces Apoptosis and Inhibits Migration in Human Colorectal Cancer Cells.
Application : WB
Reactivity : Human
Lin JH et al. Stem Cells International 2019; 2019:Article ID 7606238 Palmitic Acid Methyl Ester Induces G2/M Arrest in Human Bone Marrow-Derived Mesenchymal Stem Cells via the p53/p21 Pathway.
Application : WB
Reactivity : Human
Chen WT et al. Int J Oncol 2019; Thermal cycling as a novel thermal therapy to synergistically enhance the anticancer effect of propolis on PANC?? cells.
Application : WB
Reactivity : Human
Chueh-Hsuan Lu et al. bioRxiv 2019; (Epub) Thermal cycling-hyperthermia in combination with polyphenols, epigallocatechin gallate and chlorogenic acid, exerts synergistic anticancer effect against human pancreatic cancer PANC-1 cells.
Application : WB
Reactivity : Human
Ko PH et al. Sci Rep 2019; 9 (1):2147 Macrophage Migration Inhibitory Factor Acts as the Potential Target of a Newly Synthesized Compound, 1-(9'-methyl-3'-carbazole)-3, 4-dihydro-β-carboline.
Application : WB
Reactivity : Human
Ming-YangLee et al. European Journal of Pharmacology 2018; 839:57-65 Indirubin-3'-oxime suppresses human cholangiocarcinoma through cell-cycle arrest and apoptosis.
Application : WB
Reactivity : Human
Sikorski K et al. Nat Methods 2018; 15 (11):909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Lu WJ et al. Food Funct 2018; 9 (8):4500-4507 Licochalcone A attenuates glioma cell growth in vitro and in vivo through cell cycle arrest.
Application : WB
Reactivity : Human
Li TN et al. J Pathol 2018; (Epub) Intrahepatic HBV large surface antigen induces hepatocyte hyperploidy via failure of cytokinesis.
Application : WB
Reactivity : Human
Huang TH et al. Mol Cell 2018; 69 (5):879-892.e5 The Histone Chaperones ASF1 and CAF-1 Promote MMS22L-TONSL-Mediated Rad51 Loading onto ssDNA during Homologous Recombination in Human Cells.
Application : WB
Reactivity : Human
Yuan SY et al. Oncol Rep 2017; 37 (2):1002-1010 Escin induces apoptosis in human renal cancer cells through G2/M arrest and reactive oxygen species-modulated mitochondrial pathways.
Chin YT et al. Oncotarget 2014; 5 (24):12891-907 Anti-proliferative and gene expression actions of resveratrol in breast cancer cells in vitro.
Application : WB
Reactivity : Human
Cheung AK et al. Mol Cancer Ther 2013; 12 (8):1393-401 Polo-like kinase inhibitor Ro5203280 has potent antitumor activity in nasopharyngeal carcinoma.
Application : WB
Reactivity : Human
Chu CS et al. J Biol Chem 2011; 286 (41):35843-51 Protein kinase A-mediated serine 35 phosphorylation dissociates histone H1.4 from mitotic chromosome.
Application : WB
Reactivity : Human
Pan J et al. Oncogene 2010; 29 (47):6245-56 Suppression of cell-cycle progression by Jun dimerization protein-2 (JDP2) involves downregulation of cyclin-A2.
Application : IP
Reactivity : Mouse
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169