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CXCR4 antibody [HL2612]

Recombinant antibody KO/KD Orthogonal Overexpression
Anti-CXCR4 antibody [HL2612] used in Western Blot (WB). GTX639064
Anti-CXCR4 antibody [HL2612] used in Western Blot (WB). GTX639064
Anti-CXCR4 antibody [HL2612] used in Western Blot (WB). GTX639064
Anti-CXCR4 antibody [HL2612] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639064
Anti-CXCR4 antibody [HL2612] used in Western Blot (WB). GTX639064
Anti-CXCR4 antibody [HL2612] used in Western Blot (WB). GTX639064

Cat. No. GTX639064

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2612

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Dog
Package
100 μl ($399),
25 μl ($169)
See all CXCR4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Product Note

This antibody was raised against human CXCR4 Intracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human CXCR4. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-X-C motif chemokine receptor 4 , CD184 , D2S201E , FB22 , HM89 , HSY3RR , LAP-3 , LAP3 , LCR1 , LESTR , NPY3R , NPYR , NPYRL , NPYY3R , WHIM , WHIMS

Cellular Localization

Cell membrane,Cell junction,Early endosome,Lysosome

Background

This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CXCR4 antibody [HL2612] used in Western Blot (WB). GTX639064

GTX639064 WB Image

KO/KD Orthogonal

Non-transfected (–) and transfected (+) Jurkat whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR4 antibody [HL2612] (GTX639064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CXCR4 antibody [HL2612] used in Western Blot (WB). GTX639064

GTX639064 WB Image

Orthogonal

Boiled and unboiled Jurkat whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR4 antibody [HL2612] (GTX639064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CXCR4 antibody [HL2612] used in Western Blot (WB). GTX639064

GTX639064 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR4 antibody [HL2612] (GTX639064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CXCR4 antibody [HL2612] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639064

GTX639064 ICC/IF Image

CXCR4 antibody [HL2612] detects CXCR4 protein at cell membrane by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CXCR4 stained by CXCR4 antibody [HL2612] (GTX639064) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CXCR4 antibody [HL2612] used in Western Blot (WB). GTX639064

GTX639064 WB Image

Orthogonal Overexpression

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR4 antibody [HL2612] (GTX639064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CXCR4 antibody [HL2612] used in Western Blot (WB). GTX639064

GTX639064 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR4 antibody [HL2612] (GTX639064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations