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ERK2 antibody [GT1183]

Recombinant antibody KO/KD
Anti-ERK2 antibody [GT1183] used in Western Blot (WB). GTX00945
Anti-ERK2 antibody [GT1183] used in Western Blot (WB). GTX00945
Anti-ERK2 antibody [GT1183] used in Western Blot (WB). GTX00945

Cat. No. GTX00945

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1183

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all ERK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human ERK2.

Purification


Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 1 , ERK , ERK2 , ERT1 , MAPK1 , MAPK2 , P42MAPK , PRKM1 , PRKM2 , mitogenactivated protein kinase 1 , p38 , p40 , p41 , p41mapk , p42MAPK , P42 MAPK

Cellular Localization

Cytoplasm,Cytoskeleton,Spindle,Nucleus,Microtubule organizing center,Centrosome,Membrane,Caveola

Background

This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES

Anti-ERK2 antibody [GT1183] used in Western Blot (WB). GTX00945

GTX00945 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK2 antibody [GT1183] (GTX00945) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERK2 antibody [GT1183] used in Western Blot (WB). GTX00945

GTX00945 WB Image

KO/KD

WB analysis of wild-type (WT) and ERK2 knockout (KO) HeLa cell extracts using GTX00945 ERK2 antibody [GT1183].
Dilution : 1:1000
Laoding : 25μg

Anti-ERK2 antibody [GT1183] used in Western Blot (WB). GTX00945

GTX00945 WB Image

WB analysis of various samples using GTX00945 ERK2 antibody [GT1183].
Dilution : 1:1000
Loading : 25μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399