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FOXO4 antibody

Orthogonal
Anti-FOXO4 antibody used in Western Blot (WB). GTX50500
Anti-FOXO4 antibody used in IHC (Paraffin sections) (IHC-P). GTX50500
Anti-FOXO4 antibody used in IHC (Paraffin sections) (IHC-P). GTX50500

Cat. No. GTX50500

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all FOXO4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa. 195~199 (A-A-S-M-D) derived from human FOXO4.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168109

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

forkhead box O4 , AFX , AFX1 , MLLT7

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Database

Research Area

DATA IMAGES

Anti-FOXO4 antibody used in Western Blot (WB). GTX50500

GTX50500 WB Image

Orthogonal

WB analysis of extracts from 293 and THP1 cells using GTX50500 FOXO4 antibody.

Anti-FOXO4 antibody used in IHC (Paraffin sections) (IHC-P). GTX50500

GTX50500 IHC-P Image

Orthogonal

IHC-P analysis of human lung carcinoma tissue using GTX50500 FOXO4 antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-FOXO4 antibody used in IHC (Paraffin sections) (IHC-P). GTX50500

GTX50500 IHC-P Image

The data was published in the journal Oncotarget in 2017. PMID: 27911272

REFERENCE

Application Reference

Ryu KJ et al. Oncotarget 2017; 8 (2) : 2466-2476 FOXO4 expression is related to stem cell-like properties and resistance to treatment in diffuse large B-cell lymphoma.
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399