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GAD65 antibody [HL1187]

Recombinant antibody Overexpression
Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493
Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493
Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493
Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493
Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493
Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493
Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493
Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493
Anti-GAD65 antibody [HL1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636493

Cat. No. GTX636493

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1187

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all GAD65 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Product Note

This antibody is specific for GAD65 protein, and it does not cross-react with GAD67 protein.

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GAD65. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909995

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate decarboxylase 2 , GAD65

Cellular Localization

Cytoplasm, cytosol,Cytoplasmic vesicle,Golgi apparatus membrane

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493

GTX636493 IHC-P Image

GAD65 antibody [HL1187] detects GAD65 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog cerebellum.
GAD65 stained by GAD65 antibody [HL1187] (GTX636493) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493

GTX636493 IHC-P Image

GAD65 antibody [HL1187] detects GAD65 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat cerebellum.
GAD65 stained by GAD65 antibody [HL1187] (GTX636493) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493

GTX636493 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [HL1187] (GTX636493) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493

GTX636493 WB Image

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [HL1187] (GTX636493) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493

GTX636493 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [HL1187] (GTX636493) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493

GTX636493 IHC-P Image

GAD65 antibody [HL1187] detects GAD65 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: GAD65 stained by GAD65 antibody [HL1187] (GTX636493) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493

GTX636493 WB Image

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [HL1187] (GTX636493) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493

GTX636493 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [HL1187] (GTX636493) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-GAD65 antibody [HL1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636493

GTX636493 ICC/IF Image

GAD65 antibody [HL1187] detects GAD65 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAD65 stained by GAD65 antibody [HL1187] (GTX636493) diluted at 1:500.
Red: Tau, a Tau marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations