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Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466]

Recombinant antibody Orthogonal
Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in Western Blot (WB). GTX636940
Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636940
Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in IHC (Paraffin sections) (IHC-P). GTX636940
Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in IHC (Paraffin sections) (IHC-P). GTX636940

Cat. No. GTX636940

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1466

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all Her2 / ErbB2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

138 kDa. ( Note )

Predict Reactivity

Mouse, Bovine, Cat, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr1248 of human Her2 / ErbB2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

erb-b2 receptor tyrosine kinase 2 , CD340 , HER-2 , HER-2/neu , HER2 , MLN 19 , NEU , NGL , TKR1

Cellular Localization

Nucleus

Background

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in Western Blot (WB). GTX636940

GTX636940 WB Image

Orthogonal

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] (GTX636940) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636940

GTX636940 ICC/IF Image

Orthogonal

Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] detects Her2 / ErbB2 (phospho Tyr1248) protein at cell membrane by immunofluorescent analysis.
Sample: Mock and treated A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Her2 / ErbB2 (phospho Tyr1248) stained by Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] (GTX636940) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in IHC (Paraffin sections) (IHC-P). GTX636940

GTX636940 IHC-P Image

Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] detects Her2 / ErbB2 (phospho Tyr1248) protein by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Her2 / ErbB2 (phospho Tyr1248) stained by Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] (GTX636940) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in IHC (Paraffin sections) (IHC-P). GTX636940

GTX636940 IHC-P Image

Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] detects Her2 / ErbB2 (phospho Tyr1248) protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
Her2 / ErbB2 (phospho Tyr1248) stained by Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] (GTX636940) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations