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LDHA antibody [HL2615]

Recombinant antibody Overexpression
Anti-LDHA antibody [HL2615] used in Western Blot (WB). GTX639069
Anti-LDHA antibody [HL2615] used in Western Blot (WB). GTX639069
Anti-LDHA antibody [HL2615] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639069

Cat. No. GTX639069

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2615

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all LDHA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:20000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Product Note

This antibody is specific for human LDHA protein, and it does not cross react with human LDHB, LDHC, LDHAL6A, and LDHAL6B protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of Human LDHA.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lactate dehydrogenase A , GSD11 , HEL-S-133P , LDHM , PIG19

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008]

Database

Research Area

DATA IMAGES

Anti-LDHA antibody [HL2615] used in Western Blot (WB). GTX639069

GTX639069 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDHA antibody [HL2615] (GTX639069) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LDHA antibody [HL2615] used in Western Blot (WB). GTX639069

GTX639069 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDHA antibody [HL2615] (GTX639069) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LDHA antibody [HL2615] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639069

GTX639069 ICC/IF Image

LDHA antibody [HL2615] detects LDHA protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: LDHA stained by LDHA antibody [HL2615] (GTX639069) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations