APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human MCL1. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
MCL1 apoptosis regulator, BCL2 family member , BCL2L3 , EAT , MCL1-ES , MCL1L , MCL1S , Mcl-1 , TM , bcl2-L-3 , mcl1/EAT
Cellular Localization
Membrane,Cytoplasm,Mitochondrion,nucleoplasm
Background
This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]
Database
Research Area
DATA IMAGES
![Anti-MCL1 antibody [HL1544] used in Western Blot (WB). GTX637019](/templates/default/js/lightbox/images/loading.gif "Anti-MCL1 antibody [HL1544] used in Western Blot (WB). GTX637019")
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GTX637019 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MCL1 antibody [HL1544] (GTX637019) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX637019 IHC-P Image
MCL1 antibody [HL1544] detects MCL1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.MCL1 stained by MCL1 antibody [HL1544] (GTX637019) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637019 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MCL1 antibody [HL1544] (GTX637019) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637019 IHC-P Image
MCL1 antibody [HL1544] detects MCL1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
MCL1 stained by MCL1 antibody [HL1544] (GTX637019) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637019 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MCL1 antibody [HL1544] (GTX637019) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637019 IHC-P Image
MCL1 antibody [HL1544] detects MCL1 protein by immunohistochemical analysis.Sample: Paraffin-embedded dog tissue.MCL1 stained by MCL1 antibody [HL1544] (GTX637019) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637019 IHC-P Image
MCL1 antibody [HL1544] detects MCL1 protein by immunohistochemical analysis.Sample: Paraffin-embedded cat tissue.MCL1 stained by MCL1 antibody [HL1544] (GTX637019) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for MCL1 antibody [HL1544] (GTX637019). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MCL1 antibody [HL1544] (GTX637019). Be the first to share your experience with this product.
![Anti-MCL1 antibody [HL1544] used in Western Blot (WB). GTX637019](/upload/website/prouct_img/small/GTX637019/GTX637019_44746_20220812_WB_TPM_watermark_22081423_970.webp "Anti-MCL1 antibody [HL1544] used in Western Blot (WB). GTX637019")
![Anti-MCL1 antibody [HL1544] used in IHC (Paraffin sections) (IHC-P). GTX637019](/upload/website/prouct_img/small/GTX637019/GTX637019_44746_20221227_IHC-P_multiple_M_22122821_984.webp "Anti-MCL1 antibody [HL1544] used in IHC (Paraffin sections) (IHC-P). GTX637019")
![Anti-MCL1 antibody [HL1544] used in Western Blot (WB). GTX637019](/upload/website/prouct_img/small/GTX637019/GTX637019_T-44697_20220624_WB_M_R_22062919_608.webp "Anti-MCL1 antibody [HL1544] used in Western Blot (WB). GTX637019")
![Anti-MCL1 antibody [HL1544] used in IHC (Paraffin sections) (IHC-P). GTX637019](/upload/website/prouct_img/small/GTX637019/GTX637019_T-44697_20220624_IHC-P_22062919_337.webp "Anti-MCL1 antibody [HL1544] used in IHC (Paraffin sections) (IHC-P). GTX637019")
![Anti-MCL1 antibody [HL1544] used in Western Blot (WB). GTX637019](/upload/website/prouct_img/small/GTX637019/GTX637019_44746_20220722_WB_22072519_335.webp "Anti-MCL1 antibody [HL1544] used in Western Blot (WB). GTX637019")
![Anti-MCL1 antibody [HL1544] used in IHC (Paraffin sections) (IHC-P). GTX637019](/upload/website/prouct_img/small/GTX637019/GTX637019_44746_20230217_IHC-P_multiple_Dog_23030219_481.webp "Anti-MCL1 antibody [HL1544] used in IHC (Paraffin sections) (IHC-P). GTX637019")
![Anti-MCL1 antibody [HL1544] used in IHC (Paraffin sections) (IHC-P). GTX637019](/upload/website/prouct_img/small/GTX637019/GTX637019_44746_20230217_IHC-P_multiple_Cat_23030219_596.webp "Anti-MCL1 antibody [HL1544] used in IHC (Paraffin sections) (IHC-P). GTX637019")
![Anti-MCL1 antibody [HL1544] used in Western Blot (WB). GTX637019](/upload/website/prouct_img/normal/GTX637019/GTX637019_44746_20220812_WB_TPM_watermark_22081423_970.webp "Anti-MCL1 antibody [HL1544] used in Western Blot (WB). GTX637019")
![Anti-MCL1 antibody [HL1544] used in IHC (Paraffin sections) (IHC-P). GTX637019](/upload/website/prouct_img/normal/GTX637019/GTX637019_44746_20221227_IHC-P_multiple_M_22122821_984.webp "Anti-MCL1 antibody [HL1544] used in IHC (Paraffin sections) (IHC-P). GTX637019")
![Anti-MCL1 antibody [HL1544] used in Western Blot (WB). GTX637019](/upload/website/prouct_img/normal/GTX637019/GTX637019_T-44697_20220624_WB_M_R_22062919_608.webp "Anti-MCL1 antibody [HL1544] used in Western Blot (WB). GTX637019")
![Anti-MCL1 antibody [HL1544] used in IHC (Paraffin sections) (IHC-P). GTX637019](/upload/website/prouct_img/normal/GTX637019/GTX637019_T-44697_20220624_IHC-P_22062919_337.webp "Anti-MCL1 antibody [HL1544] used in IHC (Paraffin sections) (IHC-P). GTX637019")
![Anti-MCL1 antibody [HL1544] used in Western Blot (WB). GTX637019](/upload/website/prouct_img/normal/GTX637019/GTX637019_44746_20220722_WB_22072519_335.webp "Anti-MCL1 antibody [HL1544] used in Western Blot (WB). GTX637019")
![Anti-MCL1 antibody [HL1544] used in IHC (Paraffin sections) (IHC-P). GTX637019](/upload/website/prouct_img/normal/GTX637019/GTX637019_44746_20230217_IHC-P_multiple_Dog_23030219_481.webp "Anti-MCL1 antibody [HL1544] used in IHC (Paraffin sections) (IHC-P). GTX637019")
![Anti-MCL1 antibody [HL1544] used in IHC (Paraffin sections) (IHC-P). GTX637019](/upload/website/prouct_img/normal/GTX637019/GTX637019_44746_20230217_IHC-P_multiple_Cat_23030219_596.webp "Anti-MCL1 antibody [HL1544] used in IHC (Paraffin sections) (IHC-P). GTX637019")