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Phosphotyrosine antibody [GT0021]

Orthogonal
Anti-Phosphotyrosine antibody [GT0021] used in Western Blot (WB). GTX630143
Anti-Phosphotyrosine antibody [GT0021] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630143

Cat. No. GTX630143

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT0021

Isotype

 IgG2b

Application

 WB, ICC/IF

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all Phosphotyrosine products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:50-1:1000
Not tested in other applications.

Positive Control

Raji (1mM Pervanadate for 30min)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.16 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to Phosphotyrosine

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888184

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Phosphotyrosine antibody [GT0021] used in Western Blot (WB). GTX630143

GTX630143 WB Image

Orthogonal

Phosphotyrosine antibody detects Phosphotyrosine protein by western blot analysis. Un-treated (-) and treated (+, 1mM Pervanadate treatment for 30min) Raji whole cell extracts (5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Phosphotyrosine antibody (GTX630143) at a dilution of 1:10000.

Anti-Phosphotyrosine antibody [GT0021] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630143

GTX630143 ICC/IF Image

Phosphotyrosine antibody [GT0021] detects Phosphotyrosine protein at cytoplasm by immunofluorescent analysis.
Sample: MDA-MB-231 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Phosphotyrosine protein stained by Phosphotyrosine antibody [GT0021] (GTX630143) diluted at 1:50.
Red: paxillin, a cytoskeleton marker, stained by Paxillin antibody (GTX125891) diluted at 1:100.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169