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Phosphotyrosine antibody [IG2]

Orthogonal
Anti-Phosphotyrosine antibody [IG2] used in Western Blot (WB). GTX25592
Anti-Phosphotyrosine antibody [IG2] used in Immunoprecipitation (IP). GTX25592
Anti-Phosphotyrosine antibody [IG2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25592

Cat. No. GTX25592

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 IG2

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, IP, ELISA

Reactivity

 Species independent
Package
50 μg ($399)
See all Phosphotyrosine products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100 - 1:1000
ICC/IF 1:50 -1:200
FACS Assay dependent
IP 2 μg
ELISA 1 μg/ml
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

O-phospho-L-tyrosine and O-phospho-DL-tyramine

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Protein kinases like platelet-derived growth factor and epidermal growth factor have the ability to phosphorylate tyrosine residues of other proteins like GTPase activating protein. Protein kinases play a prominent role in the regulation of normal growth as well as tumor growth. A number of retroviruses encode proteins with tyrosine kinase activity and infection with such a virus is associated with a 10-20 fold increase in phosphotyrosine on the virally encoded protein as well as on other cellular proteins. This antibody, from clone 1G2, is capable of specifically binding to proteins which contain phosphorylated tyrosine residues, but will not react with any other phosphorylated amino acids, proteins or nuecleotides, and is excellent for the study of growth factor receptors, oncogenes, and signal transduction.

Research Area

DATA IMAGES

Anti-Phosphotyrosine antibody [IG2] used in Western Blot (WB). GTX25592

GTX25592 WB Image

Orthogonal

Tyrosine phosphorylated c-Abl and Bcr-Abl was performed following immunoprecipitation of K562 cell lysates using a c-Abl antibody, from untreated cells (left lane) or cells treated with 10uM of the tyrosine kinase inhibitor Gleevec (right lane) for 24 hours. WB was performed using GTX25592 Phosphotyrosine antibody [IG2] at a dilution of 1:250.

Anti-Phosphotyrosine antibody [IG2] used in Immunoprecipitation (IP). GTX25592

GTX25592 IP Image

Orthogonal

IP analysis of K562 cells (left lane) or cells treated with 10uM of the tyrosine kinase inhibitor Gleevec (right lane) for 24 hours using GTX25592 Phosphotyrosine antibody [IG2].
IP antibody:GTX25592
IB antibody : c-abl antibody
IP reaction : 2μg antibody / 500μg lysate

Anti-Phosphotyrosine antibody [IG2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25592

GTX25592 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX25592 Phosphotyrosine antibody [IG2].
Green : Primary antibody

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399