Phosphotyrosine antibody [RM111]

Cat. No. GTX60918

Host	Rabbit
Clonality	Monoclonal
Clone Name	RM111
Isotype	IgG
Application	WB, ICC/IF, FACS, IP, ELISA, IHC
Reactivity	Species independent
Package	100 μg ($419)

See all Phosphotyrosine products

Datasheet

View product citations for antibody GTX60918 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000 - 1:5000
ICC/IF	1:200 - 1:500
FACS	Assay dependent
IP	1:200 - 1:1000
ELISA	Assay dependent
IHC	1:200 - 1:500

Not tested in other applications.

Product Note

This antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Immunogen

Phosphotyrosine-BSA conjugate

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX60918 Image

Luminex analysis of Clone RM111’s reaction against phosphorylated or nonphosphorylated Serine, Threonine, and Tyrosine. The GTX60918 reacts only to phosphorylated Tyrosine.

GTX60918 IP Image

IP analysis of EGF-treated A431 cells using GTX60918 Phosphotyrosine antibody [RM111].
Lane 1 : Whole lysate control
Lane 2 : IP by rabbit IgG control
Lane 3 : IP by Primary antibody
Dilution : 1:1000

GTX60918 ICC/IF Image

ICC/IF analysis of A431 cells nontreated or treated with EGF using GTX60918 Phosphotyrosine antibody [RM111].
Red : Primary antibody
Blue : DAPI
Dilution : 1:500

GTX60918 WB Image

WB analysis of serum-starved A431 cells non-treated or treated with EGF lysates using GTX60918 Phosphotyrosine antibody [RM111].
Dilution : 1:5000

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 419

Qty

Phosphotyrosine antibody [RM111]

Cat. No. GTX60918

Host

Clonality

Clone Name

Isotype

Application

Reactivity