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Progesterone Receptor antibody [Alpha PR6]

Orthogonal
Anti-Progesterone Receptor antibody [Alpha PR6] used in Western Blot (WB). GTX22765
Anti-Progesterone Receptor antibody [Alpha PR6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22765
Anti-Progesterone Receptor antibody [Alpha PR6] used in IHC (Paraffin sections) (IHC-P). GTX22765

Cat. No. GTX22765

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 Alpha PR6

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay

Reactivity

 Human, Mouse, Rat, Rabbit, Sheep, Bovine, Chicken, Guinea pig, Camelid, Primate
Package
100 μg ($399)
See all Progesterone Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/ml
ICC/IF 10 μg/ml
IHC-P 5 μg/ml
IHC-Fr 20 μg/ml
IP Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Product Note

This antibody does detects the B form of the progesterone receptor. It does not cross-react with estrogen receptor or glucocorticoid receptor.

PROPERTIES

Form

Liquid

Buffer

buffer

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Chicken

Immunogen

Progesterone receptor purified from chick oviduct cytosol.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_372543

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

progesterone receptor , NR3C3

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. [provided by RefSeq, Jan 2011]

Database

Research Area

DATA IMAGES

Anti-Progesterone Receptor antibody [Alpha PR6] used in Western Blot (WB). GTX22765

GTX22765 WB Image

WB analysis of 20 ug of T47D cell lysates, untreated (-) or stimulated (+) with 100 nm promegestone for 1 hour using GTX22765 Progesterone Receptor antibody [Alpha PR6].
Dilution : 1 μg/ml

Anti-Progesterone Receptor antibody [Alpha PR6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22765

GTX22765 ICC/IF Image

Orthogonal

ICC/IF analysis of T47D cells untreated (left) or stimulated with 100nm promegestone, R5020, for 1 hour (right) using GTX22765 Progesterone Receptor antibody [Alpha PR6].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes

Anti-Progesterone Receptor antibody [Alpha PR6] used in IHC (Paraffin sections) (IHC-P). GTX22765

GTX22765 IHC-P Image

IHC-P analysis of human uterus tissue using GTX22765 Progesterone Receptor antibody [Alpha PR6].
Antigen retrieval : 10mM sodium citrate followed by microwave treatment for 8-15 minutes.
Dilution : 1:20

REFERENCE

Application Reference

Jamio? M et al. Molecules 2022; 27 (21) Effect of Sex Steroids and PGF(2α) on the Expression of Their Receptors and Decorin in Bovine Caruncular Epithelial Cells in Early-Mid Pregnancy.
Application : WB
Reactivity : Bovine
Leonel ECR et al. Ecotoxicol Environ Saf 2020; 188 : 109918 Impact of perinatal bisphenol A and 17帣 estradiol exposure: Comparing hormone receptor response.
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399