SEK1 / MKK4 antibody [8A8]

Name: SEK1 / MKK4 antibody [8A8]
Brand: GeneTex
SKU: GTX84162
Price: 519.00 USD

Anti-SEK1 / MKK4 antibody [8A8] used in Western Blot (WB). GTX84162

Anti-SEK1 / MKK4 antibody [8A8] used in IHC (Paraffin sections) (IHC-P). GTX84162

Anti-SEK1 / MKK4 antibody [8A8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84162

Anti-SEK1 / MKK4 antibody [8A8] used in Flow cytometry (FACS). GTX84162

Anti-SEK1 / MKK4 antibody [8A8] used in Immunoprecipitation (IP). GTX84162

Cat. No. GTX84162

Host	Mouse
Clonality	Monoclonal
Clone Name	8A8
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, FACS, IP
Reactivity	Human, Dog
Package	100 μl ($519)

See all SEK1 / MKK4 products

Datasheet
Document

View product citations for antibody GTX84162 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:2000
ICC/IF	1:50-1:100
IHC-P	1:150
FACS	1:100
IP	2ug/500ul

Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human MAP2K4 expression vector

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase 4 , JNKK , JNKK1 , MAPKK4 , MEK4 , MKK4 , PRKMK4 , SAPKK-1 , SAPKK1 , SEK1 , SERK1 , SKK1

Cellular Localization

Cytoplasm,Nucleus

Background

Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).

Database

Gene ID: 6416 MAP2K4
UniProt: P45985 MAP2K4

Research Area

DATA IMAGES

GTX84162 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84162 SEK1 / MKK4 antibody [8A8].
Loading : 10 ug per lane
Dilution : 1:500

GTX84162 WB Image

WB analysis of various cell lines using GTX84162 SEK1 / MKK4 antibody [8A8].
Loading : 35 ug per lane

GTX84162 IHC-P Image

IHC-P analysis of human lung tissue using GTX84162 SEK1 / MKK4 antibody [8A8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84162 IHC-P Image

IHC-P analysis of human thyroid carcinoma tissue using GTX84162 SEK1 / MKK4 antibody [8A8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84162 WB Image

WB analysis of HEK293T cells transfected with SEK1 / MKK4 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84162 SEK1 / MKK4 antibody [8A8].
Loading : 5 ug per lane

GTX84162 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84162 SEK1 / MKK4 antibody [8A8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84162 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with SEK1 / MKK4 plasmid using GTX84162 SEK1 / MKK4 antibody [8A8].

GTX84162 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX84162 SEK1 / MKK4 antibody [8A8].

GTX84162 FACS Image

FACS analysis of HEK293T cells transfected with either SEK1 / MKK4 plasmid(Red) or empty vector control plasmid(Blue) using GTX84162 SEK1 / MKK4 antibody [8A8].

GTX84162 IHC-P Image

IHC-P analysis of human thyroid tissue using GTX84162 SEK1 / MKK4 antibody [8A8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84162 IHC-P Image

IHC-P analysis of human kidney carcinoma tissue using GTX84162 SEK1 / MKK4 antibody [8A8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84162 IP Image

IP analysis of DDDDK tagged SEK1 / MKK4 overexpressed HEK293T lysate using GTX84162 SEK1 / MKK4 antibody [8A8].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

SEK1 / MKK4 antibody [8A8]

Cat. No. GTX84162

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX84162 WB Image

GTX84162 WB Image

GTX84162 IHC-P Image

GTX84162 IHC-P Image

GTX84162 WB Image

GTX84162 IHC-P Image

GTX84162 ICC/IF Image

GTX84162 ICC/IF Image

GTX84162 FACS Image

GTX84162 IHC-P Image

GTX84162 IHC-P Image

GTX84162 IP Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.