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SEK1 / MKK4 antibody [HL1839]

Recombinant antibody Orthogonal Overexpression
Anti-SEK1 / MKK4 antibody [HL1839] used in Western Blot (WB). GTX637564
Anti-SEK1 / MKK4 antibody [HL1839] used in Western Blot (WB). GTX637564
Anti-SEK1 / MKK4 antibody [HL1839] used in Western Blot (WB). GTX637564
Anti-SEK1 / MKK4 antibody [HL1839] used in IHC (Paraffin sections) (IHC-P). GTX637564
Anti-SEK1 / MKK4 antibody [HL1839] used in IHC (Paraffin sections) (IHC-P). GTX637564
Anti-SEK1 / MKK4 antibody [HL1839] used in IHC (Paraffin sections) (IHC-P). GTX637564

Cat. No. GTX637564

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1839

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SEK1 / MKK4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human SEK1 / MKK4.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase 4 , JNKK , JNKK1 , MAPKK4 , MEK4 , MKK4 , PRKMK4 , SAPKK-1 , SAPKK1 , SEK1 , SERK1 , SKK1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-SEK1 / MKK4 antibody [HL1839] used in Western Blot (WB). GTX637564

GTX637564 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1839] (GTX637564) diluted at 1:5000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SEK1 / MKK4 antibody [HL1839] used in Western Blot (WB). GTX637564

GTX637564 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1839] (GTX637564) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-SEK1 / MKK4 antibody [HL1839] used in Western Blot (WB). GTX637564

GTX637564 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1839] (GTX637564) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SEK1 / MKK4 antibody [HL1839] used in IHC (Paraffin sections) (IHC-P). GTX637564

GTX637564 IHC-P Image

SEK1 / MKK4 antibody [HL1839] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse adrenal gland.
SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1839] (GTX637564) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SEK1 / MKK4 antibody [HL1839] used in IHC (Paraffin sections) (IHC-P). GTX637564

GTX637564 IHC-P Image

SEK1 / MKK4 antibody [HL1839] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1839] (GTX637564) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SEK1 / MKK4 antibody [HL1839] used in IHC (Paraffin sections) (IHC-P). GTX637564

GTX637564 IHC-P Image

SEK1 / MKK4 antibody [HL1839] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat urinary bladder.
SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1839] (GTX637564) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations