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SEK1 / MKK4 antibody [HL1888]

Recombinant antibody KO/KD Orthogonal
Anti-SEK1 / MKK4 antibody [HL1888] used in Western Blot (WB). GTX637637
Anti-SEK1 / MKK4 antibody [HL1888] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637637
Anti-SEK1 / MKK4 antibody [HL1888] used in Western Blot (WB). GTX637637
Anti-SEK1 / MKK4 antibody [HL1888] used in Western Blot (WB). GTX637637
Anti-SEK1 / MKK4 antibody [HL1888] used in Western Blot (WB). GTX637637
Anti-SEK1 / MKK4 antibody [HL1888] used in Western Blot (WB). GTX637637

Cat. No. GTX637637

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1888

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SEK1 / MKK4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human SEK1 / MKK4. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase 4 , JNKK , JNKK1 , MAPKK4 , MEK4 , MKK4 , PRKMK4 , SAPKK-1 , SAPKK1 , SEK1 , SERK1 , SKK1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-SEK1 / MKK4 antibody [HL1888] used in Western Blot (WB). GTX637637

GTX637637 WB Image

KO/KD

Untransfected (–) and transfected (+) HeLa whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (GTX637637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SEK1 / MKK4 antibody [HL1888] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637637

GTX637637 ICC/IF Image

SEK1 / MKK4 antibody [HL1888] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1888] (GTX637637) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SEK1 / MKK4 antibody [HL1888] used in Western Blot (WB). GTX637637

GTX637637 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (GTX637637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SEK1 / MKK4 antibody [HL1888] used in Western Blot (WB). GTX637637

GTX637637 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (GTX637637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-SEK1 / MKK4 antibody [HL1888] used in Western Blot (WB). GTX637637

GTX637637 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (GTX637637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SEK1 / MKK4 antibody [HL1888] used in Western Blot (WB). GTX637637

GTX637637 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (GTX637637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations